PXD048798-1

PXD048798 is an original dataset announced via ProteomeXchange.

Title	FP-biotin enrichment of serine hydrolases in Enterococcus faecium
Description	In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of enzymes that include established drug targets like penicillin-binding proteins, have other less-studied subfamilies. In addition to fluorescent, gel-based profiling, we used a biotin-tagged fluorophosphonate probe for the enrichment and identification of serine hydrolase enzymes via streptavidin enrichment and liquid chromatography/mass spectrometry analysis. This led to the discovery of 11 largely unexplored potential targets (including α,β-hydrolases, SGNH-hydrolases, phospholipases, amidases, and peptidases) that could be exploited for drug developmentagainst the vancomycin-resistant E. faecium strain E745.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:33:19.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeanette, Slettnes Grunnvåg
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Enterococcus faecium; NCBI TaxID: 1352;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 03:06:24	ID requested
⏵ 1	2024-06-22 08:33:20	announced

10.1093/femsmc/xtae015;

Grunnv, å, g JS, Hegstad K, Lentz CS, . FEMS Microbes, 5():xtae015(2024) [pubmed]

submitter keyword: LC-MSMS,Enterococcus faecium, Activity-based protein profiling, FP-biotin, serine hydrolases

Christian Lentz
contact affiliation	Host-Microbe interactions group, the department of medical biology, UiT - The Arctic University of Norway, Tromsø, Norway.
contact email	christian.s.lentz@uit.no
lab head
Jeanette, Slettnes Grunnvåg
contact affiliation	UiT - The Arctic University of Norway
contact email	jeanette.grunnvag@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD048798

PRIDE project URI

• PRIDE
  1. PXD048798
     1. Label: PRIDE project
     2. Name: FP-biotin enrichment of serine hydrolases in Enterococcus faecium