PXD048736-2

PXD048736 is an original dataset announced via ProteomeXchange.

Title	Proteotranscriptomics of the most popular hoast sea anemone Entacmaea quadricolor reveals not all toxin genes expressed are recruited into its venom arsenal
Description	While the unique symbiotic relationship between anemonefish and sea anemones is iconic, it is still not fully understood how anemonefish withstand and thrive within this venomous host environment. In this study we used a proteotranscriptomics approach to elucidate the proteinaceous toxin repertoire from the most popular host sea anemone Entacmaea quadricolor. Although 1251 different toxin or toxin-like RNA transcripts were expressed in E.quadricolor tentacles and 2736 proteins were detected in milked venom, only 135 (approx. 10%) of proteins in venom were classified as putative toxins. This work raises the perils of defining a dominant venom type based on transcriptomics data alone in sea anemones, as we found that the dominant venom type differed between the transcriptome and proteome data. Moreover, anemonefishes interact with sea anemone proteins, so it is important when determining the dominant toxin type to examine the peptides and proteins that are present in host sea anemone venom and mucus which anemonefishes are known to interact.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:24:51.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Colella
SpeciesList	scientific name: Entacmaea quadricolor; NCBI TaxID: 6118;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-21 22:21:47	ID requested
1	2024-01-23 18:17:02	announced
⏵ 2	2024-10-22 06:24:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: sea anemone, toxin, proteotranscriptomics,venomics

Catherine Abbott
contact affiliation	College of Science and Engineering, Flinders University, Bedford Park, South Australia 5042, Australia
contact email	Catherine.abbott@flinders.edu.au
lab head
Alex Colella
contact affiliation	Flinders University
contact email	alex.colella@flinders.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048736
     1. Label: PRIDE project
     2. Name: Proteotranscriptomics of the most popular hoast sea anemone Entacmaea quadricolor reveals not all toxin genes expressed are recruited into its venom arsenal