PXD048694-1

PXD048694 is an original dataset announced via ProteomeXchange.

Title	INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double- strand breaks
Description	This study shows that Integrator complex subunit 6 (INTS6) associates with the trimeric SOSS1 (comprising INTS3, INIP, and hSSB1) to form a tetrameric SOSS1 complex following DNA damage. INTS6 binds to DNA:RNA hybrids and plays a crucial role in Protein Phosphatase 2 (PP2A) recruitment to DNA double-strand breaks (DSBs), facilitating the dephosphorylation of RNAPII. Furthermore, INTS6 prevents the accumulation of damage-induced RNA transcripts and stabilizes DNA:RNA hybrids at DSB sites. INTS6 interacts with, and promotes the recruitment of Senataxin (SETX) to DSBs, facilitating the resolution of DNA:RNA hybrids/R-loops. These data demonstrate the significance of the SOSS1 complex in autoregulating DNA:RNA dynamics and the promotion of efficient DNA repair.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:35:12.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felipe Cesar Ferrarezi Beckedorff
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-18 11:43:58	ID requested
⏵ 1	2025-05-06 12:35:13	announced

10.1093/nar/gkae937;

Long Q, Ajit K, Sedova K, Haluza V, Stefl R, Dokaneheifard S, Beckedorff F, Valencia MG, Sebesta M, Shiekhattar R, Gullerova M, Tetrameric INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double-strand breaks. Nucleic Acids Res, 52(21):13036-13056(2024) [pubmed]

submitter keyword: INTS6SETX,INTS6, INT6 complex

Ramin Shiekhattar
contact affiliation	Department of Human Genetics, University of Miami, Miller School of Medicine, Sylvester Comprehensive Cancer Center, Miami, FL, USA
contact email	rshiekhattar@miami.edu
lab head
Felipe Cesar Ferrarezi Beckedorff
contact affiliation	Department of Human Genetics, University of Miami, Miller School of Medicine, Sylvester Comprehensive Cancer Center, 1501 NW 10th Avenue, Miami, FL 33136, USA
contact email	fxf201@med.miami.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048694

PRIDE project URI

• PRIDE
  1. PXD048694
     1. Label: PRIDE project
     2. Name: INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double- strand breaks