PXD048646-1

PXD048646 is an original dataset announced via ProteomeXchange.

Title	Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells
Description	Integrins provide an essential bridge between cancer cells and the extracellular matrix, playing a central role in every stage of disease progression. Despite the recognized significance of integrin phosphorylation in several biological processes, the regulatory mechanisms and their relevance remained elusive. To address this gap, we engineered a fluorescence resonance energy transfer biosensor for integrin β1 phosphorylation, screening 96 protein tyrosine phosphatases and identifying Shp2 and PTP-PEST as negative regulators. Mutation of the integrin NPxY(783/795) sites revealed the importance of integrin phosphorylation for efficient cancer cell invasion, further supported by inhibition of the identified integrin phosphorylation regulators Shp2 and Src kinase. Using proteomics approaches, we uncovered Cofilin as a component of the phosphorylated integrin-Dok1 complex and linked this axis to effective invadopodia formation, a process supporting breast cancer invasion. These data further implicate dynamic modulation of integrin β1 phosphorylation at NPxY sites at different stages of metastatic dissemination.
HostingRepository	PRIDE
AnnounceDate	2025-02-05
AnnouncementXML	Submission_2025-02-05_01:06:50.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Conway
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 06:01:32	ID requested
⏵ 1	2025-02-05 01:06:51	announced

Dataset with its publication pending

submitter keyword: Breast cancer, phosphorylation,Integrins, extracellular matrix

Prof. Johanna
contact affiliation	Turku Bioscience Centre, University of Turku, FI-20520 Turku, Finland
contact email	joivaska@utu.fi
lab head
James Conway
contact affiliation	Turku Bioscience Centre, University of Turku, Tykistökatu 6, FI-20520 Turku, Finland
contact email	jdconw@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD048646

PRIDE project URI

• PRIDE
  1. PXD048646
     1. Label: PRIDE project
     2. Name: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells