PXD048632-1

PXD048632 is an original dataset announced via ProteomeXchange.

Title	MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms
Description	Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which are largely driven by specialized proteomes and interactomes. To define the proteome and interactome of a specific type of tissue in an intact animal, we developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase (MetRS) to label proteins with a bifunctional amino acid photo-ANA in selected cells. We applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of new tissue-specific proteins. Using the photo-crosslinker, we successfully profiled HSP90 interactors in muscles and neurons and identified tissue-specific interactors and stress-related interactors. Our study demonstrates that MACSPI can be used to profile tissue-specific proteomes and interactomes in intact multicellular organisms.
HostingRepository	PRIDE
AnnounceDate	2024-05-02
AnnouncementXML	Submission_2024-05-02_03:28:59.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	siyue huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-16 23:16:02	ID requested
⏵ 1	2024-05-02 03:29:00	announced
2	2024-10-22 06:37:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Interactomic,MACSPI, Proteomic, Photo-ANA

XIANG DAVID
contact affiliation	The University of Hong Kong
contact email	xiangli@hku.hk
lab head
siyue huang
contact affiliation	the University of Hong Kong
contact email	hugsy@hku.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD048632

PRIDE project URI

• PRIDE
  1. PXD048632
     1. Label: PRIDE project
     2. Name: MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms