PXD048613 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics analysis of blood neutrophils from WT and Wnt5 DKO mice under DSS treatment |
| Description | Neutrophils are abundant innate immune cells with important roles in host defense and many inflammatory diseases. They possess plasticity and heterogenicity, which underlie their functional ambivalency; they can be beneficial or detrimental to host well-being depending on contexts. While bone marrow is often regarded as the primary source of neutrophil production, the roles of extramedullary production in regulating neutrophil plasticity and heterogenicity in the context of autoimmune diseases remain poorly understood. Here, we report that the lack of both WNT5A and WNT5B unleashes anti-inflammatory protection in mice from Dextran Sodium Sulfate-induced colitis, accompanied with reduced colonic CD8+ T cell activation and enhanced splenic extramedullary myelopoiesis. By using the proteomics method to analyze the blood neutrophils from WT and Wnt5 DKO mice under DSS treatment, our analysis revealed elevated CD101 expression in blood neutrophils from Wnt5 DKO mice, leading to the suppression of CD8 T cell activation in vitro. This proteomic investigation unveiled the mechanism through which neutrophils from Wnt5 DKO mice inhibit CD8 T cells activation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-16 |
| AnnouncementXML | Submission_2026-03-16_04:50:14.170.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenxue Li |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-01-16 11:55:59 | ID requested | |
| ⏵ 1 | 2026-03-16 04:50:15 | announced | |
Publication List
| Luan Y, Hu J, Wang Q, Wang X, Li W, Qu R, Yang C, Rajendran BK, Zhou H, Liu P, Zhang N, Shi Y, Liu Y, Tang W, Lu J, Wu D, Wnt5 controls splenic myelopoiesis and neutrophil functional ambivalency during DSS-induced colitis. Cell Rep, 43(3):113934(2024) [pubmed] |
| 10.1016/j.celrep.2024.113934; |
Keyword List
| submitter keyword: Wnt5, Neutrophil, proteomics, DIA |
Contact List
| Yansheng Liu |
|---|
| contact affiliation | Yale Cancer Biology Institute |
| contact email | yansheng.liu@yale.edu |
| lab head | |
| Wenxue Li |
|---|
| contact affiliation | Yale University |
| contact email | wenxue.li@yale.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD048613 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048613
- Label: PRIDE project
- Name: Proteomics analysis of blood neutrophils from WT and Wnt5 DKO mice under DSS treatment
