⮝ Full datasets listing
PXD048609-1
PXD048609 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Large Aging Neutrophil-Derived Vesicles (LAND-Vs) Perpetuate Host Anti-Inflammatory Response to Facilitate Inflammation Resolution |
| Description | Precise regulation of the duration and extent of inflammation is crucial for balancing pathogen elimination and preventing tissue damage. The mechanisms underlying inflammation resolution are not yet fully understood. Here, we reveal that neutrophils, typically known for their pro-inflammatory role and pathogen clearance capabilities, can paradoxically aid in resolving inflammation by actively producing anti-inflammatory vesicles. Large aging neutrophil-derived vesicles (LAND-Vs) emerged in inflamed lungs during bacterial pneumonia. These vesicles, protected from efferocytotic clearance by phagocytes due to surface CD47, CD24, or CD31, accumulated in the resolution phase of inflammation and persisted at the site long after neutrophil recruitment subsided. CD55 on LAND-Vs exerted a robust, sustained anti-inflammatory effect by inhibiting complement 3 convertase and suppressing complement activity, thereby reducing neutrophil recruitment and tissue damage. A combination of neutrophil-specific CD55 knockout and adoptive transfer experiments demonstrated that LANDs were not only essential but also sufficient for efficient inflammation resolution. CD55+ LAND-Vs originated in lipid rafts, where CD55 accumulated asymmetrically during neutrophil aging, and subsequently formed through RhoA-dependent budding. Intriguingly, the number of CD55+ LAND-Vs in the peripheral blood of severe COVID-19 patients was markedly lower than that in healthy individuals or patients with milder cases of COVID-19, indicating that LAND-Vs might contribute to mitigating pathologies observed in severe COVID-19 patients. In conclusion, LAND-Vs, a unique structure originating from aging neutrophils, emerges as a pivotal physiological immunomodulator and showcases functions that transcend the limited lifespan of neutrophils, offering a promising therapeutic target for inflammatory and infectious diseases. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-23 |
| AnnouncementXML | Submission_2026-03-22_17:49:23.479.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alan Hsu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-16 08:55:57 | ID requested | |
| ⏵ 1 | 2026-03-22 17:49:24 | announced |
Publication List
| Hsu AY, Huang Q, Pi X, Fu J, Raghunathan K, Ghimire L, Balasubramanian A, Xie X, Yu H, Loison F, Haridas V, Zha J, Liu F, Park SY, Bagale K, Ren Q, Fan Y, Zheng Y, Cancelas JA, Chai L, Stowell SR, Chen K, Xu R, Wang X, Xu Y, Zhang L, Cheng T, Ma F, Thiagarajah JR, Wu H, Feng S, Luo HR, Neutrophil-derived vesicles control complement activation to facilitate inflammation resolution. Cell, 188(6):1623-1641.e26(2025) [pubmed] |
| 10.1016/j.cell.2025.01.021; |
Keyword List
| submitter keyword: LAND-V, human neutrophils,human vesicle |
Contact List
| Hongbo Luo | |
|---|---|
| contact affiliation | 1Department of Pathology, Dana-Farber/Harvard Cancer Center, PhD Program in Immunology, Harvard Medical School; Department of Laboratory Medicine, Boston Children's Hospital, Enders Research Building, Room 811, Boston, MA, 02115, USA |
| contact email | Hongbo.Luo@childrens.harvard.edu |
| lab head | |
| Alan Hsu | |
| contact affiliation | Boston childrens hospital |
| contact email | alan.hsu@childrens.harvard.edu |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD048609 |
| PRIDE project URI |
Repository Record List
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