PXD048508-1

PXD048508 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SIAH3 is frequently epigenetically silenced in cancer and regulates mitochondrial metabolism - SIAH3 Interactomics
Description	Of the seven in absentia homologue (SIAH) family, three members have been identified in the human genome. In contrast to the E3 ubiquitin ligase encoding SIAH1 and SIAH2, little is known on the regulation and function of SIAH3 in tumorigenesis. In this study, we reveal that SIAH3 is frequently epigenetically silenced in different cancer entities, including cutaneous melanoma, lung adenocarcinoma and head and neck cancer. Low SIAH3 levels correlate with an impaired survival of cancer patients. Additionally, induced expression of SIAH3 reduces cell proliferation. Functionally, SIAH3 negatively affects cellular metabolism by shifting cells form aerobic oxidative phosphorylation to glycolysis. SIAH3 is localized in the mitochondrion and interacts with proteins involved in mitochondrial ribosome biogenesis and translation. We also report that SIAH3 interacts with ubiquitin ligases, including SIAH1 or SIAH2, and is degraded by them. These results suggest that SIAH3 acts as an epigenetically controlled tumor suppressor by regulating cellular metabolism through the inhibition of oxidative phosphorylation.
HostingRepository	MassIVE
AnnounceDate	2024-09-09
AnnouncementXML	Submission_2024-09-09_04:24:43.336.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Graumann
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-12 06:22:33	ID requested
⏵ 1	2024-09-09 04:24:43	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SIAH, metabolism, hypermethylation, cancer, epigenetics

submitter keyword: SIAH, metabolism, hypermethylation, cancer, epigenetics

Contact List

Reinhard H. Dammann
contact affiliation	Institute for Genetics, Justus-Liebig-University Giessen, D-35392 Giessen
contact email	reinhard.dammann@gen.bio.uni-giessen.de
lab head
Johannes Graumann
contact affiliation	Philipps-University Marburg
contact email	johannes.graumann@uni-marburg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093850/