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PXD048498-1

PXD048498 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of extracellular vesicle in A549 cells under oxidative stress
DescriptionExosomes from cancer cells are versatile mediators of cell-to-cell communication, whose cargoes are dynamically loaded in response to various stress conditions. In this study, we demonstrate that under oxidative stress, cancer cells secrete exosomes that induce apoptosis in neighboring cells via the Arg/N-degron pathway. In this mechanism, RILP (Rab interacting lysosomal protein) is cleaved at Asp75 in response to oxidative stress which requires ATE1 R-transferase. The cleaved form of RILP recruits the ESCRT-II proteins VPS22 and VPS36 to endosomes from which the interluminal vesicles are invaginated generating exosomes. By using proteomics analyses, we also demonstrate exosomes secreted from cancer cells upon oxidative stress are enriched apoptotic proteins including pro-apoptotic and anti-inflammatory cytokine ANXA1. These exosomes induce apoptosis of normal cancer cells transporting ANXA1 in an Arg/N-degron pathway-dependent manner. Our results show that the Arg/N-degron pathway modulates exosome-mediated apoptosis in cancer cells under oxidative stress underlying RILP-dependent secretion of ANXA1.
HostingRepositoryPRIDE
AnnounceDate2025-04-16
AnnouncementXMLSubmission_2025-04-15_23:03:06.557.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDohyun Han
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-12 01:31:11ID requested
12025-04-15 23:03:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: A549, oxidative stress,exosome
Contact List
Dohyun Han
contact affiliationProteomics core facility, Biomedical Research Institute, Seoul National University Hospital
contact emailhdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliationProteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital
contact emailhdh03@snu.ac.kr
dataset submitter
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