PXD048450-1

PXD048450 is an original dataset announced via ProteomeXchange.

Title	Sit4 genetically interacts with Vps27 to regulate mitochondrial function and lifespan in Saccharomyces cerevisiae
Description	Sit4 is a PP2A-like Ser/Thr protein phosphatase implicated in the regulation of cellular processes important for cell survival during aging. We have previously shown that SIT4 deletion promotes vacuolar acidification, mitochondrial derepression and oxidative stress resistance, increasing yeast chronological lifespan. In this study, we performed a proteomic analysis of isolated vacuoles and yeast genetic interaction analysis to unravel how Sit4 influences vacuolar and mitochondrial function. By employing high-resolution mass spectrometry, we show that sit4Δ vacuolar membranes were enriched in Vps27 and Hse1, two proteins that are part of the endosomal sorting complex required for transport-0 complex. In addition, SIT4 exhibited a negative genetic interaction with VSP27, as sit4∆vps27∆ double mutants had a shortened lifespan compared to sit4∆ and vps27∆ single mutants. Our results also show that Vps27 did not increase sit4∆ lifespan by improving protein trafficking or vacuolar sorting pathways. However, Vps27 was critical for iron homeostasis and mitochondrial function in sit4∆ cells, as sit4∆vps27∆ double mutants exhibited high iron levels, impaired mitochondrial respiration and mitochondrial DNA (mtDNA) depletion. These findings show for the first-time a cross-talk between Sit4 and Vps27, providing new insights into the mechanisms governing chronological lifespan.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:06:05.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-11 00:23:19	ID requested
⏵ 1	2024-05-24 01:06:06	announced
2	2024-10-22 06:42:35	announced	2024-10-22: Updated project metadata.

10.3390/cells13080655;

Martins TS, Correia M, Pinheiro D, Lemos C, Mendes MV, Pereira C, Costa V, . Cells, 13(8):(2024) [pubmed]

submitter keyword: Sit4

Vps27

vacuolar trafficking

mitochondria

iron

chronological aging

Vítor Costa
contact affiliation	1- i3S - Instituto de Investigação e Inovação em Saúde,Universidade do Porto, Portugal 2- IBMC - Instituto de Biologia Celular e Molecular, Universidade do Porto, Portugal 3- ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Portugal
contact email	vcosta@ibmc.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048450
     1. Label: PRIDE project
     2. Name: Sit4 genetically interacts with Vps27 to regulate mitochondrial function and lifespan in Saccharomyces cerevisiae