PXD048449-1

PXD048449 is an original dataset announced via ProteomeXchange.

Title	The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium
Description	Bacterial small proteins impact diverse physiological processes, however, technical challenges posed by small size hampered their systematic identification and biochemical characterization. In our quest to uncover small proteins relevant for Salmonella pathogenicity, we previously identified YjiS, a 54 amino acid protein, which is strongly induced during this pathogen’s intracellular infection stage and exhibits features of a virulence suppressor. Here, we set out to further characterize the virulence-related role of YjiS. Cell culture infection assays with Salmonella mutants lacking or overexpressing YjiS corroborated previous functional genomics data and additionally suggested this small protein to counteract bacterial escape from macrophages. Mutant scanning of the protein’s conserved, arginine-rich DUF1127 domain excluded a major effect of single amino acid substitutions on the infection phenotype. A comparative dual RNA-seq assay uncovered the molecular footprint of YjiS in the macrophage response to infection, with effects related to oxidative stress and endo-/exocytosis. Bacterial cell fractionation experiments demonstrated YjiS to associate with the inner membrane, without being itself membrane-inserted. Accordingly, proteins interacting with YjiS in pull-down experiments were enriched for inner membrane processes. Together, our study points to a role for Salmonella YjiS as a membrane-associated suppressor of pathogen dissemination.
HostingRepository	PRIDE
AnnounceDate	2024-12-12
AnnouncementXML	Submission_2024-12-12_02:02:06.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium; NCBI TaxID: 90371;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-11 00:17:09	ID requested
⏵ 1	2024-12-12 02:02:06	announced

10.1093/FEMSML/UQAE026;

submitter keyword: Salmonella Typhimurium, DUF1127,YjiS, macrophage, dual RNA-seq, mass spectrometry, host-pathogen interaction, small protein

Dörte Becher
contact affiliation	Institute of Microbiology, Department of Microbial Proteomics, University of Greifswald, D-17489, Greifswald, Germany
contact email	Dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048449
     1. Label: PRIDE project
     2. Name: The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium