PXD048382-1

PXD048382 is an original dataset announced via ProteomeXchange.

Title	An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly
Description	Mechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 (DHX15 in humans) to productively remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered segments that organize Prp43 function during ribosome assembly. In addition to the activating G-patch, we discovered an inhibitory segment within Tma23 and Pxr1, I-patch, that restrains Prp43 ATPase activity. Cryo-electron microscopy and hydrogen-deuterium exchange mass spectrometry show how I-patch binds to the catalytic RecA-like domains to allosterically inhibit Prp43 ATPase activity. Tma23 and Pxr1 contain dimerization segments that organize Prp43 into higher-order complexes. We posit that Prp43 function at discrete locations on pre-ribosomal RNA is coordinated through toggling interactions with G-patch and I-patch segments. This could guarantee measured and timely Prp43 activation, enabling precise control over multiple RNA remodelling events occurring concurrently during ribosome formation.
HostingRepository	PRIDE
AnnounceDate	2024-10-09
AnnouncementXML	Submission_2024-10-09_05:23:28.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Portugal
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 04:27:50	ID requested
⏵ 1	2024-10-09 05:23:28	announced

Dataset with its publication pending

submitter keyword: Ribosome assembly, mass spectrometry, Prp43, RNA helicase

Vikram Panse
contact affiliation	Institute of Medical Microbiology, University of Zürich
contact email	vpanse@imm.uzh.ch
lab head
Daniela Portugal
contact affiliation	University of Zürich
contact email	dportugal@imm.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD048382

PRIDE project URI

• PRIDE
  1. PXD048382
     1. Label: PRIDE project
     2. Name: An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly