PXD048338-1

PXD048338 is an original dataset announced via ProteomeXchange.

Title	The effect of the production system on the proteomics profiles of the Longissimus thoracis muscle in Arouquesa cattle
Description	Arouquesa breed is an autochthonous Portuguese breed produced under a traditional mountain system. It is known for its Arouquesa PDO beef, a high-quality product with valued organoleptic characteristics. There is a need to improve production systems without affecting beef quality. The aim of this work is to compare the proteomics profile from the Longissimus thoracis muscle from Arouquesa animals produced under different systems. Sixty weaners were produced under the following systems: traditional (TF) and traditional with starter feed supplementation (TF+S1) with weaning and slaughtering at 8 months, the S1+S2 (weaning at 5 months and grower supplement until slaughter) and two rearing periods with finishing supplementation (TF+S3 and S3). Upon slaughter, samples of L. thoracis were taken and analyzed using a shotgun proteomics workflow. Several putative biomarkers of beef quality for the Arouquesa breed were identified: VIM, FSCN1, SERPINH1, ALDH1A1, NDUFB5, ANXA1, PDK4, CEMIP2, NDUFB9, PDLIM1, OXCT1, MYH4. These proteins were involved in actin binding, skeletal muscle development and in the mitochondrial respiratory chain and they can influence mostly meat tenderness and color. Our results demonstrate the link between production practices and putative meat quality signatures, that have potential to improve traceability of certified products.
HostingRepository	PRIDE
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2024-08-08_10:39:20.248.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antje Dittmann
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-09 03:39:10	ID requested
⏵ 1	2024-08-08 10:39:21	announced
2	2024-10-22 06:53:26	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2024.105265;

Sacarr, ã, o-Birrento L, Ribeiro DM, Dittmann A, Alves SP, Kunz L, Silva S, Ven, â, ncio CA, de Almeida AM, The effect of the production system on the proteomics profiles of the Longissimus thoracis muscle in Arouquesa cattle. J Proteomics, 307():105265(2024) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: shotgun proteomics, beef quality, differentially abundant proteins,Autochthonous beef breeds, environmental sustainability

André M. de Almeida
contact affiliation	LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
contact email	aalmeida@isa.ulisboa.pt
lab head
Antje Dittmann
contact affiliation	UZH
contact email	antje.dittmann@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD048338

PRIDE project URI

• PRIDE
  1. PXD048338
     1. Label: PRIDE project
     2. Name: The effect of the production system on the proteomics profiles of the Longissimus thoracis muscle in Arouquesa cattle