PXD048315-1

PXD048315 is an original dataset announced via ProteomeXchange.

Title	Response and recovery of biogas-producing microbiomes after loading disturbances with microalgae as feedstock
Description	Industrial anaerobic digestion (AD) represents a relevant energy source beyond today’s fossil fuels, wherein organic matter is recycled to methane gas via an intricate and complex microbial food web. Despite its potential, anaerobic reactors often undergo process instability over time, mainly caused by substrate composition perturbations, making the system unreliable for stable energy production. To ensure the reliability of AD technologies, it is crucial to identify microbial- and system responses to better understand the effect of such perturbations and ultimately detect signatures indicative of process failure . Here, we investigate the effect of microalgal organic loading rate (OLR) on the fermentation products profile, microbiome dynamics, and disruption/recovery of major microbial metabolisms. Reactors subjected to low- and high-OLR disturbances were operated and monitored for fermentation products and biogas production over time, while microbial responses were investigated via 16S rRNA gene amplicon data, shotgun metagenomics and metagenome-centric metaproteomics.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_02:14:41.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Archaea; NCBI TaxID: 2157; scientific name: Bacteria; NCBI TaxID: 2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-08 07:51:30	ID requested
⏵ 1	2025-02-21 02:14:41	announced

10.1128/MSYSTEMS.01674-24;

submitter keyword: Methane, anaerobic digestion, metaproteomics

Magnus Øverlie Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048315
     1. Label: PRIDE project
     2. Name: Response and recovery of biogas-producing microbiomes after loading disturbances with microalgae as feedstock