PXD048267-1

PXD048267 is an original dataset announced via ProteomeXchange.

Title	Proteome labeled by a cuprous ion-responsive protein labeling reagent in the ATP7A WT and ATP7A KO living cells
Description	We have developed a new molecular probe called CuR. This probe is selectively activated by labile Cu(I), hence enabling labeling of the proteins in Cu(I)-rich cellular environments. We applied CuR to ATP7A-WT and ATP7A-KO cells that were pretreated with or without Cu(gtsm). Next, we enriched the labeled proteins by immunoprecipitation, digested them with trypsin, and analyzed them using liquid chromatography-tandem mass spectrometry. We used label-free quantification in the mass spectrometry analysis to detect changes in labeled proteins caused by exogenous copper compared to the non-copper-treated condition.
HostingRepository	jPOST
AnnounceDate	2025-01-05
AnnouncementXML	Submission_2025-01-04_07:00:03.971.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rong Cheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-05 00:51:34	ID requested
⏵ 1	2025-01-04 07:00:04	announced

Dataset with its publication pending

submitter keyword: Copper ions, Chemical proteomics, Cu(I)-responsive, ATP7A

Itaru Hamachi
lab head
Rong Cheng
contact affiliation	Kyoto University
dataset submitter

jPOST dataset URI

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