PXD048246-1

PXD048246 is an original dataset announced via ProteomeXchange.

Title	The PRC1 and PRC2 proximal interactome: the link between H4ac an H3K27me3
Description	Polycomb Repressive Complexes PRC1 and PRC2 play a crucial role in silencing lineage-specific genes during early embryogenesis. To provide new insights in polycomb biology, we profiled the proximal interactome (proxeome) of the catalytic subunits RNF2 (PRC1) and EZH2 (PRC2) in mouse embryonic stem cells (mESCs). This revealed >100 proteins proximal to PRC12 and PRC21, which mainly comprise transcription factors, transcriptional regulators and RNA binding proteins. To investigate the link between PRC2 and NuA4, and therefore the the link between H4ac an H3K27me3, we perturbed PRC2 both chemically and genetically.The effects on the histone PTMs were measured with LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-02-25
AnnouncementXML	Submission_2025-02-25_02:37:11.854.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048246
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maarten Dhaenens
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monomethylated residue; crotonylated residue; trimethylated residue; acetylated residue; monohydroxylated residue; formylated residue; dimethylated residue; deamidated residue; butanoylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-03 23:56:33	ID requested
⏵ 1	2025-02-25 02:37:12	announced

10.6019/PXD048246;

10.17632/TDGZFZ4HTZ.2;

submitter keyword: H3K27me3, PTMs,PRC1, PRC2, H4ac, Histones, LC-MS/MS

Dr Maarten Dhaenens
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium
contact email	maarten.dhaenens@ugent.be
lab head
Maarten Dhaenens
contact affiliation	Faculity of Pharmaceutical Biotechnology
contact email	maarten.dhaenens@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD048246

PRIDE project URI

• PRIDE
  1. PXD048246
     1. Label: PRIDE project
     2. Name: The PRC1 and PRC2 proximal interactome: the link between H4ac an H3K27me3