PXD048215-1

PXD048215 is an original dataset announced via ProteomeXchange.

Title	Autophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Tandem Affinity Purification coupled to LC-MS/MS of SFB-TLK1 133-208 and 133-208 Y149A F150A)
Description	Tandem affinity purification of HEK 293T cells transiently expressing SFB-TLK1 133-208 or SFB-TLK1 133-208 Y149A F150A to identify if TLK1 PIP box mutants can still interact with PCNA.
HostingRepository	PRIDE
AnnounceDate	2025-01-02
AnnouncementXML	Submission_2025-01-02_07:20:17.044.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048215
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kirk West
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-02 20:50:25	ID requested
⏵ 1	2025-01-02 07:20:17	announced

10.6019/PXD048215;

10.1093/nar/gkae1279;

West KL, Nguyen TTN, Tengler KA, Kreiling N, Raney KD, Ghosal G, Leung JW, Autophosphorylation of the Tousled-like kinases TLK1 and TLK2 regulates recruitment to damaged chromatin via PCNA interaction. Nucleic Acids Res, ():(2024) [pubmed]

submitter keyword: Tandem Affinity purification,Tousled-Like Kinases, LC-MS/MS

Justin W. Leung
contact affiliation	Department of Radiation Oncology, University of Texas Health Sciences Center at San Antonio
contact email	leungJ@uthscsa.edu
lab head
Kirk West
contact affiliation	University of Arkansas for Medical Sciences
contact email	kwest@uams.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD048215

PRIDE project URI

• PRIDE
  1. PXD048215
     1. Label: PRIDE project
     2. Name: Autophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Tandem Affinity Purification coupled to LC-MS/MS of SFB-TLK1 133-208 and 133-208 Y149A F150A)