PXD048200-1

PXD048200 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomics to study CHN-1/HSP-1 complex substrate turnover in C. elegans
Description	The wild type (WT) and HSP-1 EEYD (worms generating endogenously mutated HSP-1) C. elegans were cultured under normal conditions (20 degrees) or exposed to 30 degrees for 16 hours as L4 larvae (referred to as heat stress/HS). In WT worms undergoing HS, the activity of CHN-1 ubiquitin ligase is anticipated to be inhibited by its interaction with wild-type HSP-1. In contrast, HSP-1 EEYD is expected not to inhibit CHN-1 in mutant worms, consequently leading to the degradation of a specific pool of proteins. This dataset unveils the identities of these proteins.
HostingRepository	PRIDE
AnnounceDate	2024-11-08
AnnouncementXML	Submission_2024-11-08_02:44:47.719.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-02 03:30:51	ID requested
⏵ 1	2024-11-08 02:44:48	announced

10.1016/J.JBC.2024.107864;

submitter keyword: E3 ligase/chaperone,C. elegans, CHN-1 (human ortholog CHIP), HSP-1 (human ortholog HSP-70)

Wojciech Pokrzywa
contact affiliation	Laboratory of Protein Metabolism, International Institute of Molecular and Cell Biology in Warsaw, 4 Ks. Trojdena Street, 02-109 Warsaw, Poland
contact email	wpokrzywa@iimcb.gov.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD048200

PRIDE project URI

• PRIDE
  1. PXD048200
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomics to study CHN-1/HSP-1 complex substrate turnover in C. elegans