PXD048194 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Native Purification of yeast Met30 in the absence and presence of Cadmium followed by DSSO crosslinking and Mass spec analysis |
Description | HBTHMet30 cells were cultured in YEP + 2% galactose + 4µM Biotin at 30˚C and treated with 100 µM CdCl2 for 30 minutes. Native whole cells lysates were prepared in lysis buffer (50mM Hepes pH7.5, 200mM NaCl, 10% glycerol, 40mM imidazole, 0.2% Triton1mM dithiothreitol, 0.1mM orthovanadate, 1mM phenylmethylsulfonyl floride [PMSF], and 1mg/ml each leupeptin and pepstatin) and bound to Ni-resin. Proteins were eluted in the presence of 250mM imidazole. For cross-linking analysis, eluted HBTHMet30 was first bound to Streptavidin beads and then on-bead cross-linked with 0.5 mM DSSO in PBS buffer for 1 h at 37 °C. Bead-bound proteins were reduced with TCEP and alkylated with iodocetamide, digested by trypsin and cleaned-up with C18 tips prior to LC MSn analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-22 |
AnnouncementXML | Submission_2024-06-22_08:35:21.178.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Clinton Yu |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-01 22:01:39 | ID requested | |
⏵ 1 | 2024-06-22 08:35:22 | announced | |
Publication List
10.1038/s41467-024-48184-6; |
Lauinger L, Andronicos A, Flick K, Yu C, Durairaj G, Huang L, Kaiser P, Cadmium binding by the F-box domain induces p97-mediated SCF complex disassembly to activate stress response programs. Nat Commun, 15(1):3894(2024) [pubmed] |
Keyword List
submitter keyword: SCFMet30, Met30 intra-crosslinks, DSSO, Mass spectrometry, Cadmium |
Contact List
Peter Kaiser |
contact affiliation | Department of Biological Chemistry, University of California Irvine, CA, USA |
contact email | pkaiser@uci.edu |
lab head | |
Clinton Yu |
contact affiliation | University of California, Irvine |
contact email | clinton.yu@uci.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD048194 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048194
- Label: PRIDE project
- Name: Native Purification of yeast Met30 in the absence and presence of Cadmium followed by DSSO crosslinking and Mass spec analysis