PXD048176-1

PXD048176 is an original dataset announced via ProteomeXchange.

Title	A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy; whole cell proteome
Description	Cells convert complex metabolic information into stress-adapted autophagy responses. Canonically, multilayered protein kinase networks converge on the conserved Atg1/ULK kinase complex (AKC) to induce non-selective and selective forms of autophagy in response to metabolic changes. Here, we show that, upon phosphate starvation, the metabolite sensor Pho81 interacts with the adaptor subunit Atg11 at the AKC via an Atg11/FIP200 interaction motif to modulate pexophagy by virtue of its conserved phospho-metabolite sensing SPX domain. Notably, core AKC components Atg13 and Atg17 are dispensable for phosphate starvation-induced autophagy revealing significant compositional and functional plasticity of the AKC. Our data indicate that, instead of functioning as a selective autophagy receptor, Pho81 compensates for partially inactive Atg13 by promoting Atg11-phosphorylation by Atg1 critical for pexophagy during phosphate starvation. Our work shows Atg11/FIP200 adaptor subunits not only bind selective autophagy receptors but also modulator subunits that convey metabolic information directly to the AKC for autophagy regulation.
HostingRepository	PRIDE
AnnounceDate	2023-12-31
AnnouncementXML	Submission_2023-12-30_21:26:21.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-29 20:44:39	ID requested
⏵ 1	2023-12-30 21:26:22	announced
2	2024-10-22 06:18:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy, TMT

Martin Graef
contact affiliation	Department of Molecular Biology and Genetics Cornell University 201 Biotechnology 526 Campus Road Ithaca, NY 14853
contact email	mg2255@cornell.edu
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD048176

PRIDE project URI

• PRIDE
  1. PXD048176
     1. Label: PRIDE project
     2. Name: A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy; whole cell proteome