PXD048172-1

PXD048172 is an original dataset announced via ProteomeXchange.

Title	Identification of Protein Targets of S-nitroso-Coenzyme A-Mediated S-Nitrosation Using Chemoproteomics
Description	S-nitrosation is a cysteine post-translational modification (PTM) fundamental to cellular signaling. This modification regulates protein function in numerous biological processes in the nervous, cardiovascular, and immune systems. Small molecule or protein nitrosothiols act as mediators of NO signaling by transferring the NO group (formally NO+) to a free thiol on a target protein through a transnitrosation reaction. The protein targets of specific transnitrosating agents and the extent and functional effects of S-nitrosation on these target proteins have been poorly characterized. S-nitroso-Coenzyme A (CoA-SNO) was recently identified as a mediator of endogenous S-nitrosation. Here, we identified direct protein targets of CoA-SNO-mediated transnitrosation using a competitive chemical-proteomic approach that quantified the extent of modification on 789 cysteine residues in response to CoA-SNO. A subset of cysteines displayed high susceptibility to modification by CoA-SNO, including previously uncharacterized sites of S-nitrosation. We further validated and functionally characterized the functional effects of S-nitrosation on the protein targets phosphofructokinase, platelet type, ATP citrate synthase, and ornithine aminotransferase.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:11:01.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Falco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-29 12:48:59	ID requested
⏵ 1	2024-10-08 11:11:01	announced
2	2024-10-22 06:19:09	announced	2024-10-22: Updated project metadata.

10.1021/ACSCHEMBIO.3C00654;

submitter keyword: S-Nitrosation

Chemoproteomics

S-Nitroso-Coenzyme A

Eranthie Weerapana
contact affiliation	Department of Chemistry, Boston College, Chestnut Hill, Massachusetts, USA
contact email	Eranthie@bc.edu
lab head
Julia Falco
contact affiliation	Boston College Massachusetts General Hospital
contact email	juliaafalco@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD048172

PRIDE project URI

• PRIDE
  1. PXD048172
     1. Label: PRIDE project
     2. Name: Identification of Protein Targets of S-nitroso-Coenzyme A-Mediated S-Nitrosation Using Chemoproteomics