PXD048172 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Protein Targets of S-nitroso-Coenzyme A-Mediated S-Nitrosation Using Chemoproteomics |
Description | S-nitrosation is a cysteine post-translational modification (PTM) fundamental to cellular signaling. This modification regulates protein function in numerous biological processes in the nervous, cardiovascular, and immune systems. Small molecule or protein nitrosothiols act as mediators of NO signaling by transferring the NO group (formally NO+) to a free thiol on a target protein through a transnitrosation reaction. The protein targets of specific transnitrosating agents and the extent and functional effects of S-nitrosation on these target proteins have been poorly characterized. S-nitroso-Coenzyme A (CoA-SNO) was recently identified as a mediator of endogenous S-nitrosation. Here, we identified direct protein targets of CoA-SNO-mediated transnitrosation using a competitive chemical-proteomic approach that quantified the extent of modification on 789 cysteine residues in response to CoA-SNO. A subset of cysteines displayed high susceptibility to modification by CoA-SNO, including previously uncharacterized sites of S-nitrosation. We further validated and functionally characterized the functional effects of S-nitrosation on the protein targets phosphofructokinase, platelet type, ATP citrate synthase, and ornithine aminotransferase. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:11:01.433.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julia Falco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Discovery |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-29 12:48:59 | ID requested | |
⏵ 1 | 2024-10-08 11:11:01 | announced | |
2 | 2024-10-22 06:19:09 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: S-Nitrosation |
Chemoproteomics |
S-Nitroso-Coenzyme A |
Contact List
Eranthie Weerapana |
contact affiliation | Department of Chemistry, Boston College, Chestnut Hill, Massachusetts, USA |
contact email | Eranthie@bc.edu |
lab head | |
Julia Falco |
contact affiliation | Boston College Massachusetts General Hospital |
contact email | juliaafalco@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048172
- Label: PRIDE project
- Name: Identification of Protein Targets of S-nitroso-Coenzyme A-Mediated S-Nitrosation Using Chemoproteomics