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PXD048158-1

PXD048158 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSuccinylation proteome analysis of Cd-exposed Neuro-2a cells
DescriptionCadmium is a neurotoxic compound which induces cognitive alterations similar to those produced by Alzheimer's disease (AD). Autophagic flux impairment is a hallmark in Cd-induced AD-like pathology. However, the underlying mechanism remains obscure. In these study, Neuro-2a cells were exposed to different concentrations of Cd (1, 2, and 4 μM) for 72 h, and C57 mice (8 week) were orally administered Cd for 12 weeks. Chronic Cd exposure inhibited autophagosome-lysosome fusion and impaired lysosomal function, thereby contributing to defects in autophagic clearance and subsequently leading to APP accumulation and nerve cell death. Mechanistically, proteomic analysis coupled with Ingenuity Pathway Analysis identified the crucial key target molecule in Cd-impaired autophagic flux. Four-dimensional label-free succinylation quantitative proteomics were performed in Neuro-2a cells with and without 4 μM CdCl2 treatment for 72h. Then, vital molecules and crucial succinylation sites were identified. Moreover, the function and important role of the resulting molecule with crucial succinylation site were determined. These results may thus open an avenue for therapeutic intervention in chronic Cd-induced AD-like pathology.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_05:06:07.642.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHuifeng Pi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListN6-succinyl-L-lysine; iodoacetic acid derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-28 11:01:52ID requested
12024-10-17 05:06:08announced
Publication List
10.1002/advs.202402030;
Deng P, Fan T, Gao P, Peng Y, Li M, Li J, Qin M, Hao R, Wang L, Li M, Zhang L, Chen C, He M, Lu Y, Ma Q, Luo Y, Tian L, Xie J, Chen M, Xu S, Zhou Z, Yu Z, Pi H, SIRT5-Mediated Desuccinylation of RAB7A Protects Against Cadmium-Induced Alzheimer's Disease-Like Pathology by Restoring Autophagic Flux. Adv Sci (Weinh), 11(30):e2402030(2024) [pubmed]
Keyword List
submitter keyword: Succinylation proteome analysis
Cd
Neuro-2a cells
Contact List
Huifeng Pi
contact affiliationThird Military Medical University
contact emailpihuifeng2010@163.com
lab head
Huifeng Pi
contact affiliationThird Military Medical University
contact emailpihuifeng2010@163.com
dataset submitter
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Dataset FTP location
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