PXD048158 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Succinylation proteome analysis of Cd-exposed Neuro-2a cells |
| Description | Cadmium is a neurotoxic compound which induces cognitive alterations similar to those produced by Alzheimer's disease (AD). Autophagic flux impairment is a hallmark in Cd-induced AD-like pathology. However, the underlying mechanism remains obscure. In these study, Neuro-2a cells were exposed to different concentrations of Cd (1, 2, and 4 μM) for 72 h, and C57 mice (8 week) were orally administered Cd for 12 weeks. Chronic Cd exposure inhibited autophagosome-lysosome fusion and impaired lysosomal function, thereby contributing to defects in autophagic clearance and subsequently leading to APP accumulation and nerve cell death. Mechanistically, proteomic analysis coupled with Ingenuity Pathway Analysis identified the crucial key target molecule in Cd-impaired autophagic flux. Four-dimensional label-free succinylation quantitative proteomics were performed in Neuro-2a cells with and without 4 μM CdCl2 treatment for 72h. Then, vital molecules and crucial succinylation sites were identified. Moreover, the function and important role of the resulting molecule with crucial succinylation site were determined. These results may thus open an avenue for therapeutic intervention in chronic Cd-induced AD-like pathology. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-17 |
| AnnouncementXML | Submission_2024-10-17_05:06:07.642.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Huifeng Pi |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | N6-succinyl-L-lysine; iodoacetic acid derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-12-28 11:01:52 | ID requested | |
| ⏵ 1 | 2024-10-17 05:06:08 | announced | |
Publication List
| 10.1002/advs.202402030; |
| Deng P, Fan T, Gao P, Peng Y, Li M, Li J, Qin M, Hao R, Wang L, Li M, Zhang L, Chen C, He M, Lu Y, Ma Q, Luo Y, Tian L, Xie J, Chen M, Xu S, Zhou Z, Yu Z, Pi H, SIRT5-Mediated Desuccinylation of RAB7A Protects Against Cadmium-Induced Alzheimer's Disease-Like Pathology by Restoring Autophagic Flux. Adv Sci (Weinh), 11(30):e2402030(2024) [pubmed] |
Keyword List
| submitter keyword: Succinylation proteome analysis |
| Cd |
| Neuro-2a cells |
Contact List
| Huifeng Pi |
|---|
| contact affiliation | Third Military Medical University |
| contact email | pihuifeng2010@163.com |
| lab head | |
| Huifeng Pi |
|---|
| contact affiliation | Third Military Medical University |
| contact email | pihuifeng2010@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD048158 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048158
- Label: PRIDE project
- Name: Succinylation proteome analysis of Cd-exposed Neuro-2a cells
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