PXD048096-2

PXD048096 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Impact of fibrillar collagen scaffold on corneal stromal fibroblasts protein expression
Description	The human cornea relies on its extracellular matrix (ECM) properties, with corneal stromal fibroblasts (CSFs) crucial for ECM remodeling. We studied the effects of nanofibrillar materials on CSFs using a decellularized human amniotic membrane (dHAM) and fibrillar collagen scaffold (FCS). FCS enhances focal adhesions (FAs), activating mechanotransduction signals through FAK autophosphorylation and YAP1 nuclear translocation. Nanofibrillar topography induces gene expression changes related to tissue remodeling and collagen production. Here, we performed shotgun proteomics analysis (DDA-PASEF in TimsToF Pro) to analyze the impact of FCS topography on CSFs protein expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:41.993.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048096
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-23 09:52:17	ID requested
1	2024-09-02 04:03:46	announced
⏵ 2	2024-10-22 06:57:45	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD048096;
10.3390/ijms25168601;
Zhurenkov KE, Lobov AA, Bildyug NB, Alexander-Sinclair EI, Darvish DM, Lomert EV, Kriger DV, Zainullina BR, Chabina AS, Khorolskaya JI, Perepletchikova DA, Blinova MI, Mikhailova NA, Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates. Int J Mol Sci, 25(16):(2024) [pubmed]

10.6019/PXD048096;

10.3390/ijms25168601;

Zhurenkov KE, Lobov AA, Bildyug NB, Alexander-Sinclair EI, Darvish DM, Lomert EV, Kriger DV, Zainullina BR, Chabina AS, Khorolskaya JI, Perepletchikova DA, Blinova MI, Mikhailova NA, Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates. Int J Mol Sci, 25(16):(2024) [pubmed]

Keyword List

submitter keyword: fibrillar collagen scaffold, TimsToF Pro, DDA-PASEF, corneal keratocytes, focal adhesion, extracellular matrix

submitter keyword: fibrillar collagen scaffold, TimsToF Pro, DDA-PASEF, corneal keratocytes, focal adhesion, extracellular matrix

Contact List

Natalia Mikhailova
contact affiliation	Cell Technologies Center, Institute of Cytology RAS,St. Petersburg, Russia
contact email	natalia.mikhailova@incras.ru
lab head
Arseniy Lobov
contact affiliation	Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD048096
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD048096

PRIDE project URI

Repository Record List

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