PXD048078-1

PXD048078 is an original dataset announced via ProteomeXchange.

Title	Ubiquitome profiling uncovers disturbed mitochondrial remodeling as an outcome of doxorubicin-induced cardiotoxicity in old mice
Description	The urgent need to understand the molecular modulation associated with chronic cardiotoxicity of doxorubicin (DOX) has prompted us to investigate the ubiquitome profile of aged cardiac muscle. Using old CD-1 male mice administered with a DOX dosage established to induce cardiotoxicity, we performed a comprehensive analysis of the proteomic profile of the enriched pool of poly-ubiquitinated proteins obtained from cardiac muscle using tandem ubiquitin-binding entities (TUBEs). GeLC-MS/MS and subsequent bioinformatic analysis revealed several proteins with the poly-ubiquitination modification involved in DOX-induced cardiotoxicity. Increased poly-ubiquitination levels were found for sarcomeric proteins including alpha-actinin-2 and desmin as well as mitochondrial proteins such as ATP synthase subunit beta and cytochrome b-c1 complex subunit 1. Thus, impaired protein ubiquitination emerges as an enduring consequence of DOX-induced cardiotoxicity. The present exploratory analysis could be considered an important starting point for further studies targeting molecular pathways under the side effects of the widely used anticancer drug DOX.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:07:32.189.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Vitorino
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-22 05:19:11	ID requested
⏵ 1	2025-06-29 16:07:33	announced

Brand, ã, o SR, Lazzari E, Vitorino R, Meroni G, Reis-Mendes A, Neuparth MJ, Amado F, Carvalho F, Ferreira R, Costa VM, Comprehensive ubiquitome analysis reveals persistent mitochondrial remodeling disruptions from doxorubicin-induced cardiotoxicity in aged CD-1 male mice. Arch Toxicol, 99(6):2447-2462(2025) [pubmed]

10.1007/s00204-025-04006-2;

submitter keyword: mitochondrial dynamics,old, chronic cardiotoxicity, ubiquitination, anticancer therapy, cardio-oncology

Rui Vitorino
contact affiliation	Instituto de Biomedicina - iBiMED Universidade de Aveiro 3810-193 Aveiro Portugal
contact email	rvitorino@ua.pt
lab head
Rui Vitorino
contact affiliation	Universidade de Aveiro
contact email	rvitorino@ua.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048078
     1. Label: PRIDE project
     2. Name: Ubiquitome profiling uncovers disturbed mitochondrial remodeling as an outcome of doxorubicin-induced cardiotoxicity in old mice