PXD048052-1

PXD048052 is an original dataset announced via ProteomeXchange.

Title	A zwitterionic detergent and catalyst-based single-cell proteomics using a loss-free microhole-collection disc
Description	Recent advances in single-cell proteomics have solved many bottlenecks, such as throughput, sample recovery, and scalability via nano-scale sample handling. In this study, we aimed for a sensitive mass spectrometry analysis capable of handling single cells with a conventional mass spectrometry workflow without additional equipment. We achieved seamless cell lysis and TMT-labeling in a micro-HOLe Disc (microHOLD single-cell proteomics) by developing a mass-compatible single solution based on a zwitterionic detergent and a catalyst for single-cell lysis and tandem mass tag-labeling without a heat incubation step. This method was developed to avoid peptide loss by surface absorption and buffer or tube changes by collecting tandem mass tag-labeled peptide through microholes placed in the liquid chromatography injection vials in a single solution. We successfully applied the microHOLD single-cell proteomics method for the analysis of proteome reprogramming in hormone-sensitive prostate cells to develop castration-resistant prostate cancer cells. This novel single-cell proteomic method is not limited by cutting-edge nano-volume handling equipment and achieves high-throughput and ultrasensitive proteomic analysis of limited samples, such as single cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-28
AnnouncementXML	Submission_2025-05-28_08:31:33.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaemyung Jang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 10:12:18	ID requested
⏵ 1	2025-05-28 08:31:34	announced

10.1021/acs.analchem.4c00158;

Yeo S, Jang J, Jung HJ, Lee H, Lee S, Choe Y, A Zwitterionic Detergent and Catalyst-Based Single-Cell Proteomics Using a Loss-Free Microhole-Collection Disc. Anal Chem, 96(29):11690-11698(2024) [pubmed]

submitter keyword: zwitterionic detergent, Tandem Mass Tags, quadrol,single-cell proteomics

Youngshik Choe
contact affiliation	Korea Brain Research Institute, Daegu, 41068, Korea
contact email	dallarae@kbri.re.kr
lab head
Jaemyung Jang
contact affiliation	Korea Brain Research Institute
contact email	piloter2@kbri.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048052

PRIDE project URI

• PRIDE
  1. PXD048052
     1. Label: PRIDE project
     2. Name: A zwitterionic detergent and catalyst-based single-cell proteomics using a loss-free microhole-collection disc