PXD048043-1

PXD048043 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Schreiber_2023_VS1_Exploring options for proximity-dependent biotinylation experiments
Description	This dataset consists of 52 raw mass spectrometry files and associated peak lists and results files, acquired on a Thermo Orbitrap Exploris 480 operated in Data-Dependent Acquisition mode. Samples were generated by Karl Schreiber (clones were generated by Eileigh Kadijk and Karl Schreiber) and affinity purification and mass spectrometric acquisition were performed by Karl Schreiber. Analysis was performed by Karl Schreiber and Ji-Young Youn. The files are associated with a manuscript by Karl Schreiber et al. that evaluates different labeling enzymes and affinity resins for proximity-dependent biotinylation experiments. Ji-Young Youn is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (jiyoung.youn@sickkids.ca). This submission is associated with three Supplementary Files (in addition to this README file): Table I describes the composition of this dataset (sample information) Table II lists all the peptide identification evidence (per MSFragger) Table III lists the SAINTexpress interactions for all analyses performed
HostingRepository	MassIVE
AnnounceDate	2024-03-04
AnnouncementXML	Submission_2024-03-04_20:14:08.131.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ji-Young Youn
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; N-acetylated residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 07:13:11	ID requested
⏵ 1	2024-03-04 20:14:08	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: proximity-dependent biotinylation, BioID, miniTurbo, APEX2, TDP-43, TARDBP, Streptavidin Sepharose, MagResyn MS, SAINTexpress, proximal interactions

submitter keyword: proximity-dependent biotinylation, BioID, miniTurbo, APEX2, TDP-43, TARDBP, Streptavidin Sepharose, MagResyn MS, SAINTexpress, proximal interactions

Contact List

Ji-Young Youn
contact affiliation	Hospital for Sick Children
contact email	jiyoung.youn@sickkids.ca
lab head
Ji-Young Youn
contact affiliation	Hospital for Sick Children
contact email	jiyoung.youn@sickkids.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093705/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093705/