PXD048031-1
PXD048031 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pyrophosphoproteomics in HCT116 wildtype vs PPIP5K1/2 knockout |
Description | In vitro, both 5PP-InsP5 (IP7) and 1,5(PP)2InsP4 (IP8) can pyrophosphorylate peptides and proteins. It is unclear, which inositol pyrophosphate species is responsible for installing endogenous pyrophosphorylation in cells. In order to test this, we used HCT116 cells deficient in IP8 synthesis. Both enzymes that convert IP7 to IP8 are knocked out in this cell line. Importantly, this cell line does overaccumulates IP7, complicating the analysis. We found no obvious difference in the number of observable pyrophosphorylation sites between the knockout and wildtype, suggesting that either IP7 or both molecules are responsible for creating pyrophosphoproteins in cells. |
HostingRepository | jPOST |
AnnounceDate | 2024-03-12 |
AnnouncementXML | Submission_2024-03-12_07:29:47.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dorothea Fiedler |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; unknown modification |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-21 01:06:29 | ID requested | |
⏵ 1 | 2024-03-12 07:29:48 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: pyrophosphorylation, inositol pyrophosphates, EThcD, PTMs, non-canonical phosphorylation |
Contact List
Dorothea Fiedler | |
---|---|
lab head | |
Dorothea Fiedler | |
contact affiliation | Leibniz Institut für Molekulare Pharmakologie, Berlin |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002429/ |