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PXD048031-1

PXD048031 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePyrophosphoproteomics in HCT116 wildtype vs PPIP5K1/2 knockout
DescriptionIn vitro, both 5PP-InsP5 (IP7) and 1,5(PP)2InsP4 (IP8) can pyrophosphorylate peptides and proteins. It is unclear, which inositol pyrophosphate species is responsible for installing endogenous pyrophosphorylation in cells. In order to test this, we used HCT116 cells deficient in IP8 synthesis. Both enzymes that convert IP7 to IP8 are knocked out in this cell line. Importantly, this cell line does overaccumulates IP7, complicating the analysis. We found no obvious difference in the number of observable pyrophosphorylation sites between the knockout and wildtype, suggesting that either IP7 or both molecules are responsible for creating pyrophosphoproteins in cells.
HostingRepositoryjPOST
AnnounceDate2024-03-12
AnnouncementXMLSubmission_2024-03-12_07:29:47.784.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDorothea Fiedler
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; unknown modification
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-21 01:06:29ID requested
12024-03-12 07:29:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: pyrophosphorylation, inositol pyrophosphates, EThcD, PTMs, non-canonical phosphorylation
Contact List
Dorothea Fiedler
lab head
Dorothea Fiedler
contact affiliationLeibniz Institut für Molekulare Pharmakologie, Berlin
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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