PXD048031-1

PXD048031 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Pyrophosphoproteomics in HCT116 wildtype vs PPIP5K1/2 knockout
Description	In vitro, both 5PP-InsP5 (IP7) and 1,5(PP)2InsP4 (IP8) can pyrophosphorylate peptides and proteins. It is unclear, which inositol pyrophosphate species is responsible for installing endogenous pyrophosphorylation in cells. In order to test this, we used HCT116 cells deficient in IP8 synthesis. Both enzymes that convert IP7 to IP8 are knocked out in this cell line. Importantly, this cell line does overaccumulates IP7, complicating the analysis. We found no obvious difference in the number of observable pyrophosphorylation sites between the knockout and wildtype, suggesting that either IP7 or both molecules are responsible for creating pyrophosphoproteins in cells.
HostingRepository	jPOST
AnnounceDate	2024-03-12
AnnouncementXML	Submission_2024-03-12_07:29:47.784.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dorothea Fiedler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; unknown modification
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 01:06:29	ID requested
⏵ 1	2024-03-12 07:29:48	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: pyrophosphorylation, inositol pyrophosphates, EThcD, PTMs, non-canonical phosphorylation

submitter keyword: pyrophosphorylation, inositol pyrophosphates, EThcD, PTMs, non-canonical phosphorylation

Contact List

Dorothea Fiedler
lab head
Dorothea Fiedler
contact affiliation	Leibniz Institut für Molekulare Pharmakologie, Berlin
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002429/