PXD047978-1

PXD047978 is an original dataset announced via ProteomeXchange.

Title	Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics
Description	Aristolochic acid nephropathy (AAN) is a kidney injury syndrome caused by aristolochic acids exposure, with unknown mechanisms and therapeutic targets. Our study used label-free quantitative proteomics to delineate renal protein profiles and identify key proteins after exposure to different doses of aristolochic acid I (AAI). Male C57BL/6 mice received AAI (1.25 mg/kg/d, 2.5 mg/kg/d, or 5 mg/kg/d) or vehicle for 5 days. The results showed that AAI induced dose-dependent nephrotoxicity. Differences in renal protein profiles between the control and AAI groups increased with AAI dose. Comparing the control with the low-, medium-, and high-dose AAI groups, we found 58, 210, and 271 differentially expressed proteins (DEPs), respectively. Furthermore, protein-protein interaction network analysis identified acyl-CoA synthetase medium-chain family member 3 (Acsm3), cytochrome P450 family 2 subfamily E member 1 (Cyp2e1), microsomal glutathione S-transferase 1 (Mgst1), and fetuin B (Fetub) as the key proteins. Proteomics revealed that AAI decreased Acsm3 and Cyp2e1 while increasing Mgst1 and Fetub expression in mice kidneys, which was further confirmed by Western blotting. Collectively, in AAI-induced nephrotoxicity, renal protein profiles were dysregulated and exacerbated with increasing AAI dose. Acsm3, Cyp2e1, Mgst1, and Fetub may be the potential therapeutic targets for AAN.
HostingRepository	iProX
AnnounceDate	2023-12-20
AnnouncementXML	Submission_2023-12-19_19:51:37.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinhui Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-19 19:51:05	ID requested
⏵ 1	2023-12-19 19:51:37	announced

Dataset with its publication pending

submitter keyword: Aristolochic acid I, Nephrotoxicity, Aristolochic acid nephropathy, Proteomics, Label-free

Xinhui Liu
contact affiliation	Shenzhen Traditional Chinese Medicine Hospital
contact email	liuxinhui0317@163.com
lab head
Xinhui Liu
contact affiliation	Shenzhen Traditional Chinese Medicine Hospital
contact email	huahun212@163.com
dataset submitter

iProX dataset URI