PXD047905-1

PXD047905 is an original dataset announced via ProteomeXchange.

Title	• SURF2 is a new key player in nucleolar stress response through the regulation of 5S RNP/MDM2/p53 axis.
Description	Cancer cells are addicted to strong ribosome production to sustain their proliferation rate. Many chemotherapies impede ribosome production which is perceived by cells as "nucleolar stress" (NS), triggering TP53-dependent and independent response pathways leading to cell cycle arrest and/or apoptosis. The 5S RNP particle, a sub-ribosomal particle, is instrumental to NS response. Upon ribosome assembly defects, the 5S RNP accumulate as free form. This free form is able to sequester and inhibit MDM2, thus promoting TP53 stabilization. To investigate how cancer cells could resist to NS, we purified free-5S RNP and uncovered a new partner, SURF2. Functional characterization of this protein shows that SURF2 depletion increases cells sensitivity to NS, while its overexpression promotes their resistance to it. Consistently, SURF2 expression level negatively correlates with the overall survival in some cancers. Our data demonstrate that SURF2 can buffer free-5S RNP particles, and modulate their activity. SURF2 appears as a new regulator of NS response and a key player in both ribosomopathies and oncogenic mechanisms.
HostingRepository	PRIDE
AnnounceDate	2024-09-30
AnnouncementXML	Submission_2024-09-30_00:44:06.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Marcoux
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-18 07:26:52	ID requested
⏵ 1	2024-09-30 00:44:06	announced

10.1038/S41467-024-52659-X;

submitter keyword: cancer, ribosome, nucleolar stress,human

Julien Marcoux
contact affiliation	1) Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France 2) Infrastructure nationale de protéomique, ProFI, FR 2048, Toulouse, France
contact email	julien.marcoux@ipbs.fr
lab head
Julien Marcoux
contact affiliation	IPBS
contact email	julien.marcoux@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD047905

PRIDE project URI

• PRIDE
  1. PXD047905
     1. Label: PRIDE project
     2. Name: • SURF2 is a new key player in nucleolar stress response through the regulation of 5S RNP/MDM2/p53 axis.