PXD047856-2

PXD047856 is an original dataset announced via ProteomeXchange.

Title	Exploring the extracellular landscape of cyanobacteria with deep exoproteomics
Description	Extracellular proteins are involved in a remarkable number of fundamental processes in cyanobacteria. Yet, there is limited knowledge regarding the identity and function of these proteins. Here, we introduce a solid-phase enhanced protein aggregation workflow that enables description of the cyanobacterial exoproteome with unprecedented depth. Application to cyanobacteria from three distinct habitats, Synechocystis sp. PCC 6803, Synechcoccus sp. PCC 11901 and Nostoc punctiforme PCC 73102, allowed the identification of up to 62% of all predicted secreted proteins. The approach was then extended to compare the Synechocystis sp. PCC 6803 wild-type secretome with that of a bloom-like aggregated state and a secretion-impaired mutant. Finally, we demonstrate that the workflow can be miniaturized and adapted to a 96-well format for high-throughput secretome analysis. Collectively, these findings challenge the general belief that cyanobacteria lack secretory proteins and point to a functional conservation of the secretome across species from different environments. Our approach can be applied to microbes from a wide range of habitats, with the potential to open new avenues of investigation in microbial exoproteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:58.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Meier
SpeciesList	scientific name: Picosynechococcus sp. PCC 11901; NCBI TaxID: 2579791; scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; scientific name: Nostoc punctiforme (strain ATCC 29133 / PCC 73102); NCBI TaxID: 63737;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-15 07:37:03	ID requested
1	2024-08-29 05:53:25	announced
⏵ 2	2024-10-22 06:56:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics,Cyanobacteria, diaPASEF

Florian Meier
contact affiliation	Functional Proteomics, University Klinikum Jena
contact email	florian.meier@med.uni-jena.de
lab head
Florian Meier
contact affiliation	Jena University Hospital
contact email	florian.meier@med.uni-jena.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD047856

PRIDE project URI

• PRIDE
  1. PXD047856
     1. Label: PRIDE project
     2. Name: Exploring the extracellular landscape of cyanobacteria with deep exoproteomics