PXD047834-1

PXD047834 is an original dataset announced via ProteomeXchange.

Title	Endogenous aryl hydrocarbon receptor ligands-dysregulated phosphoproteomes and proteomes in human fetal endothelial cells
Description	Preeclampsia (PE) is a leading cause of maternal and fetal morbidity and mortality and is characterized by a wide spectrum of impaired maternal and fetal vascular function. Aryl hydrocarbon receptor (AhR, a ligand-activated transcription factor) plays a critical role in regulating vascular development and function. Endogenous AhR ligands can induce endothelial dysfunction. However, the underlying protein phospho-signaling mechanisms remain unknown. To determine if endogenous AhR ligands dysregulate the phosphoproteomes and proteomes in endothelial cells, primary human umbilical vein endothelial cells (HUVECs) (n = 4; 2 cell preparations/cell sex) were cultured in endothelial cell media (ECM). After 16 hr serum starvation, subconfluent cells were treated with 1 µM 2-(1’H-indole-3’-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE, an endogenous AhR ligand) or DMSO (vehicle) for 4 and 24 hr. The cell proteins were subjected to a bottom-up phosphoproteomic analysis to determine acute and prolonged effects of ITE on protein phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_13:28:27.299.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047834
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Si-yan Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-14 10:20:18	ID requested
⏵ 1	2025-02-10 13:28:29	announced

10.6019/PXD047834;

Zhao YJ, Zhang SY, Wei YY, Li HH, Lei W, Wang K, Kumar S, Zhou C, Zheng J, An endogenous aryl hydrocarbon receptor ligand dysregulates endothelial functions, transcriptome, and phosphoproteome. Am J Physiol Cell Physiol, 328(3):C954-C966(2025) [pubmed]

10.1152/ajpcell.00849.2024;

submitter keyword: Phosphoproteomics, Endothelial cells, Preeclampsia,AhR ligands

Jing Zheng
contact affiliation	Department of Obstetrics and Gynecology, University of Wisconsin-Madison, WI,USA.
contact email	jzheng@wisc.edu
lab head
Si-yan Zhang
contact affiliation	Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA.
contact email	szhang767@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047834
     1. Label: PRIDE project
     2. Name: Endogenous aryl hydrocarbon receptor ligands-dysregulated phosphoproteomes and proteomes in human fetal endothelial cells