PXD047824 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Oleate grown bioreactor sludge metaproteome 1 |
Description | A combination of shotgun metaproteomics and 16S rRNA gene pyrosequencing wasused to identify potential functional pathways and key microorganisms involved in long-chain fatty acids (LCFA) anaerobic conversion. Microbial communities degrading saturated- and unsaturated-LCFA were compared. Archaeal communities were mainly composed of Methanosaeta, Methanobacterium and Methanospirillum species, both in stearate (saturated C18:0) and oleate (mono-unsaturated C18:1) incubations. Over 80% of the 16S rRNA gene sequences clustered within the Methanosaeta genus, which is in agreement with the high number of proteins assigned to this group (94%). Archaeal proteins related with methane metabolism were highly expressed. Bacterial communities were rather diverse and the composition dissimilar between incubations with saturated- and unsaturated-LCFA. Stearate-degrading communities were enriched in Deltaproteobacteria (34% of the assigned sequences), while microorganisms clustering within the Synergistia class were more predominant in oleate incubation (25% of the assigned sequences). Bacterial communities were diverse and active, given by the high percentage of proteins related with mechanisms of energy production. Several proteins were assigned to syntrophic bacteria, emphasizing the importance of the interactions between acetogens and methanogens in energy exchange and formation in anaerobic LCFA-rich environments. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-03 |
AnnouncementXML | Submission_2024-05-03_08:39:04.235.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD047824 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | João Sequeira |
SpeciesList | scientific name: bioreactor sludge metagenome; NCBI TaxID: 412754; scientific name: cellular organisms; NCBI TaxID: NCBITaxon:131567; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Dionex instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-14 04:11:45 | ID requested | |
⏵ 1 | 2024-05-03 08:39:04 | announced | |
Publication List
Keyword List
submitter keyword: bioreactor sludge metaproteome, fatty acids degradation, oleate |
Contact List
Joao Carlos Sequeira |
contact affiliation | Centre of Biological Engineering, Minho University |
contact email | jsequeira@ceb.uminho.pt |
lab head | |
João Sequeira |
contact affiliation | Centre of Biological Engineering |
contact email | maildosequeira@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047824
- Label: PRIDE project
- Name: Oleate grown bioreactor sludge metaproteome 1