PXD047795-2

PXD047795 is an original dataset announced via ProteomeXchange.

Title	Nitrogen signaling factor triggers a respiration-like gene expression program
Description	Microbes have evolved intricate communication systems that enable individual cells of a population to send and receive signals in response to changes in their immediate environment. In the fission yeast Schizosaccharomyces pombe, nitrogen signaling factor (NSF) is part of such communication system, which functions to regulate the usage of different nitrogen sources. Yet, the pathways and mechanisms by which NSF acts are poorly understood. Here, we show that NSF physically interacts with the mitochondrial sulfide quinone oxidoreductase Hmt2 and that it prompts a change from a fermentation- to a respiration-like gene expression program independently of the carbon source. Our results suggest that NSF activity is not restricted to nitrogen metabolism alone and that it could function as a rheostat to prepare a population of S. pombe cells for an imminent shortage of their preferred nutrients.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:26.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vytautas Iesmantavicius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-13 08:46:36	ID requested
1	2024-06-25 11:11:47	announced
⏵ 2	2024-10-22 06:47:27	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: NSF, cell communication, nitrogen metabolism, nitrogen signaling

Marc Bühler
contact affiliation	Professor in Molecular Biology & Research Group Leader, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
contact email	marc.buehler@fmi.ch
lab head
Vytautas Iesmantavicius
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	vytautas.iesmantavicius@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD047795

PRIDE project URI

• PRIDE
  1. PXD047795
     1. Label: PRIDE project
     2. Name: Nitrogen signaling factor triggers a respiration-like gene expression program