PXD047760-1

PXD047760 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Pleiotropic effects of PhaR regulator in Bradyrhizobium diazoefficiens microaerobic metabolism
Description	Bradyrhizobium diazoefficiens can live inside soybean root nodules and in free-living conditions. In both states, when oxygen levels decrease, cells adjust their protein pools by gene transcription modulation. PhaR encodes a transcription factor annotated as PHA (polyhydroxyalkanoate) accumulation regulator. We found that PhaR not only controls the PHA cycle but also acts as a global regulator of excess carbon allocation by controlling the expression of fixK2 and nifA genes, both encoding key transcription factors for microoxic and symbiotic metabolism in B. diazoefficiens. The function of PhaR was expanded by a multi-pronged approach that includes analysis of the effects of phaR mutation at transcriptional and protein levels of putative PhaR targets and direct control mediated by PhaR determined by EMSA assays. We also were able to identify PhaR, phasins and other proteins associated with PHA granules which confirmed a global function of PhaR in microoxia.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_02:09:03.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Ignacio Quelas
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bradyrhizobium japonicum USDA 110; NCBI TaxID: 224911;
ModificationList	No PTMs are included in the dataset
Instrument	Xcalibur

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-12 09:06:24	ID requested
⏵ 1	2024-05-22 02:09:04	announced
2	2024-10-22 06:41:13	announced	2024-10-22: Updated project metadata.

Publication List

Quelas JI, Cabrera JJ, D, í, az-Pe, ñ, a R, S, á, nchez-Schneider L, Jim, é, nez-Leiva A, Tortosa G, Delgado MJ, Pettinari MJ, Lodeiro AR, Del Val C, Mesa S, Microaerobic Metabolism. Int J Mol Sci, 25(4):(2024) [pubmed]
10.3390/ijms25042157;

Quelas JI, Cabrera JJ, D, í, az-Pe, ñ, a R, S, á, nchez-Schneider L, Jim, é, nez-Leiva A, Tortosa G, Delgado MJ, Pettinari MJ, Lodeiro AR, Del Val C, Mesa S, Microaerobic Metabolism. Int J Mol Sci, 25(4):(2024) [pubmed]

10.3390/ijms25042157;

Keyword List

submitter keyword: polyhydroxybutyrate, proteomics,microoxia, protein-DNA interaction, Rhizobia, transcriptomics

submitter keyword: polyhydroxybutyrate, proteomics,microoxia, protein-DNA interaction, Rhizobia, transcriptomics

Contact List

Juan Ignacio Quelas
contact affiliation	Instituto de Biotecnología y Biología Molecular (IBBM-CONICET). La Plata, Buenos Aires, Argentina.
contact email	juan.i.quelas@gmail.com
lab head
Juan Ignacio Quelas
contact affiliation	Instituto de Biotecnología y Biología Molecular
contact email	jquelas@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047760
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047760

PRIDE project URI

Repository Record List

[ + ]