PXD047750-2

PXD047750 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics identification of Listeria monocytogenes T cell epitopes in mice
Description	Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8 T-cell responses, but very few T cell epitopes are known in mice as most common animal infection model for listeriosis. To identify epitopes we screened for Listeria epitopes presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics. In between more than 6,000 mouse self-peptides presented on MHC Class I molecules, we detected 26 Listeria peptides from 25 different bacterial proteins, including previously reported antigens. Bacterial immunopeptides with confirmed fragmentation spectra were further tested for their potential to CD8 T cells, revealing VTYNYINI from the putative cell wall surface anchor family protein LMON_0576 as a novel bona fide peptide epitope. Despite its high biological potency in a prime boost model, this epitope did not protect against challenge infection but can be used as a research tool to probe CD8 T cell responses in mouse models of Listeria infection. Our results demonstrate the power of immunopeptidomics for bacterial antigen identification but highlight the need for in-depth immune characterization for vaccine candidate selection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:55.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Willems
SpeciesList	scientific name: Listeria monocytogenes; NCBI TaxID: NCBITaxon:1639; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-12 05:11:53	ID requested
1	2024-08-13 03:20:27	announced
⏵ 2	2024-10-22 06:54:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: MHC,Listeria monocytogenes, T-cell epitopes, immunopeptidomics, mouse

Francis Impens
contact affiliation	Ghent University, Faculty of Medicine and Health Sciences, Deparment of Biomolecular Medicine
contact email	Francis.Impens@UGent.be
lab head
Patrick Willems
contact affiliation	Ghent University
contact email	patwille.willems@ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047750
     1. Label: PRIDE project
     2. Name: Immunopeptidomics identification of Listeria monocytogenes T cell epitopes in mice