PXD047725-1

PXD047725 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling reveals dynamic changes in the SQSTM1 protein network
Description	Sequestosome1 (SQSTM1) is an autophagy receptor that mediates degradation of intracellular cargo, including protein aggregates, through multiple protein interactions. These interactions form the SQSTM1 protein network that are mediated by SQSTM1 functional interaction domains, which include LIR, PB1, UBA and KIR. Despite various attempts to unravel the complexity of the SQSTM1 protein network, our understanding of the relationship of various components in cellular physiology and disease states continues to evolve. To investigate the SQSTM1 protein interaction network, we performed proximity profile labeling by fusing TurboID with the human protein SQSTM1 (TurboID::SQSTM1). This chimeric protein displayed well-established SQSTM1 features including: production of SQSTM1 intracellular bodies, binding to known SQSTM1 interacting partners via defined functional SQSTM1 interacting domains and capture of novel SQSTM1 interactors. Strikingly, aggregated tau protein altered the protein interaction network of SQSTM1 to include many stress associated proteins. Overall, our work reveals the dynamic landscape of the SQSTM1 protein network and offers a resource to study SQSTM1 function in cellular physiology and disease state.
HostingRepository	PRIDE
AnnounceDate	2024-08-06
AnnouncementXML	Submission_2024-08-06_01:11:30.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Rondon Ortiz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 10:40:09	ID requested
⏵ 1	2024-08-06 01:11:31	announced
2	2024-10-22 06:52:35	announced	2024-10-22: Updated project metadata.

10.1016/J.JBC.2024.107621;

submitter keyword: autophagy, TurboID, proximity labeling, tau, protein aggregation, p62,SQSTM1, MAPT

Ben Wolozin
contact affiliation	Departments of Anatomy & Neurobiology, Boston University, Boston, MA 02215, USA Center for Systems Neuroscience, Boston University, Boston, MA 02115, USA Center for Neurophotonics, Boston University, Boston, MA 02115, USA Department of Neurology, Boston University, Boston, MA 02115, USA
contact email	bwolozin@bu.edu
lab head
Alejandro Rondon Ortiz
contact affiliation	Boston University
contact email	arortiz@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD047725

PRIDE project URI

• PRIDE
  1. PXD047725
     1. Label: PRIDE project
     2. Name: Proximity labeling reveals dynamic changes in the SQSTM1 protein network