PXD047724-1

PXD047724 is an original dataset announced via ProteomeXchange.

Title	Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation
Description	Tardigrades can survive remarkable doses of ionizing radiation, up to about 1000 times the lethal dose for humans. How they do so is incompletely understood. We found that the tardigrade Hypsibius exemplaris suffers DNA damage upon gamma irradiation, but damage is repaired. We show that tardigrades have a specific and robust response to ionizing radiation: irradiation induces a rapid, dramatic upregulation of many DNA repair genes. By expressing tardigrade genes in bacteria, we validate that increased expression of some repair genes can suffice to increase radiation tolerance. We show that at least one such gene is necessary for tardigrade radiation tolerance. Tardigrades’ ability to sense ionizing radiation and massively upregulate specific DNA repair pathway genes may represent an evolved solution for maintaining DNA integrity.
HostingRepository	PRIDE
AnnounceDate	2024-12-13
AnnouncementXML	Submission_2024-12-13_08:17:58.237.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047724
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Hypsibius exemplaris; NCBI TaxID: 2072580;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 10:38:03	ID requested
⏵ 1	2024-12-13 08:17:59	announced

10.6019/PXD047724;

Clark-Hachtel CM, Hibshman JD, De Buysscher T, Stair ER, Hicks LM, Goldstein B, The tardigrade Hypsibius exemplaris dramatically upregulates DNA repair pathway genes in response to ionizing radiation. Curr Biol, 34(9):1819-1830.e6(2024) [pubmed]

10.1016/j.cub.2024.03.019;

submitter keyword: LC-MSMS,Tardigrades, transciptomics

Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill, Department of Chemistry
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047724
     1. Label: PRIDE project
     2. Name: Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation