PXD047720-1

PXD047720 is an original dataset announced via ProteomeXchange.

Title	VAPB interacts with phosphorylated ELYS at decondensing chromosomes during mitosis (AP-MS Data)
Description	ELYS is a nucleoporin that localizes to the nuclear side of the nuclear pore complex (NPC) in interphase cells. In mitosis, it serves as an assembly platform that interacts with chromatin and then with nucleoporin subcomplexes to initiate post-mitotic NPC assembly. Here we identified and characterized ELYS as a major binding partner of the membrane protein VAPB during mitosis. In mitosis, ELYS becomes phosphorylated at many sites, including a predicted FFAT (two phenylalanines in an acidic tract) motif, which is shown to mediate interaction with the MSP (major sperm protein)-domain of VAPB. Phosphorylation-dependent binding of VAPB to ELYS is demonstrated by peptide binding assays and co-immunoprecipitation experiments. In anaphase, the two proteins co-localize to the non-core region of the newly forming nuclear envelope. Depletion of VAPB resulted in prolonged mitosis and slow progression from meta- to anaphase and also to chromosome segregation defects. Together, our results suggest a role of ELYS-VAPB-complexes in recruiting membrane fragments to chromatin.
HostingRepository	PRIDE
AnnounceDate	2024-04-12
AnnouncementXML	Submission_2024-04-12_07:33:48.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 09:16:18	ID requested
⏵ 1	2024-04-12 07:33:49	announced
2	2024-10-22 06:35:58	announced	2024-10-22: Updated project metadata.

10.1038/S44319-024-00125-6;

submitter keyword: ELYS, nucleoporin, chromosome segregation, mitosis, nuclear pore complex

Christof Lenz
contact affiliation	University Medical Center Goettingen, Department of Clinical Chemistry, Core Facility Proteomics
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD047720

PRIDE project URI

• PRIDE
  1. PXD047720
     1. Label: PRIDE project
     2. Name: VAPB interacts with phosphorylated ELYS at decondensing chromosomes during mitosis (AP-MS Data)