PXD047714-1

PXD047714 is an original dataset announced via ProteomeXchange.

Title	A comprehensive proteomics analysis of the hemolymph composition of sugar-fed Aedes aegypti female and male mosquitoes
Description	In arthropods, hemolymph carries immune cells and solubilizes and transport nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1,403 proteins were identified, 447 of them predicted to be extracellular. In both sexes almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundance (based on their intensity based absolute quantification, iBAQ) were 37.9% and 33.2%, respectively. Interestingly, among them 102 serine proteases/serine protease-homologs were identified, almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, like odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of Ae. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on the hemolymph composition.
HostingRepository	PRIDE
AnnounceDate	2026-07-06
AnnouncementXML	Submission_2026-07-06_09:13:23.116.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Silva
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: NEWT:7159;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 07:59:20	ID requested
⏵ 1	2026-07-06 09:13:23	announced

10.1021/acs.jproteome.3c00918;

Alvarenga PH, Alves E Silva TL, Suzuki M, Nardone G, Cecilio P, Vega-Rodriguez J, Ribeiro JMC, Andersen JF, Female and Male Mosquitoes. J Proteome Res, 23(4):1471-1487(2024) [pubmed]

submitter keyword: A comprehensive proteomics analysis of the hemolymph composition of sugar-fed Aedes aegypti female and male mosquitoes

Jose Marcos Chaves Ribeiro
contact affiliation	National Institutes of Health
contact email	jribeiro@niaid.nih.gov
lab head
Thiago Silva
contact affiliation	National Institutes of Health
contact email	thiagoluiz.alvesesilva@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD047714

PRIDE project URI

• PRIDE
  1. PXD047714
     1. Label: PRIDE project
     2. Name: A comprehensive proteomics analysis of the hemolymph composition of sugar-fed Aedes aegypti female and male mosquitoes