PXD047703-1

PXD047703 is an original dataset announced via ProteomeXchange.

Title	0518_4 Fission yeast Paa1 interactome
Description	We performed mass-spectrometry analysis of proteins co-purifying with the Paa1, the structural subunit of the PP2A protein phosphatase complex. Paa1 was tagged with YFP at the C-terminus and expressed from its endogenous promoter at its chromosomal locus. After one hour of nitrogen starvation, Paa1-YFP was pulled down and co-purifying proteins were analysed by mass-spectrometry. Our results revealed the presence of all components of PP2A protein complexes including Paa1, the catalytic subunits Ppa1, Ppa2, and Ppa3, and the regulatory subunits Pab1, Par1 and Par2. Additionally, several PP2A regulators (Igo1, Zds1, Dis2, Ppe1 and Ekc1) and components of the PP2A SIP/STRIPAK complex were also detected, confirming that the pull-down approach was successful. The mass-spectrometry analysis also showed an over-representation of proteins related to ribosome structure, translation initiation, aminoacylation, and tRNA modification in the Paa1 interactome. We focused on Trm112, a widely conserved protein with a crucial role in translation.
HostingRepository	PRIDE
AnnounceDate	2024-12-02
AnnouncementXML	Submission_2024-12-02_00:42:24.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Moreno
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NCBITaxon:4896; scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 03:13:21	ID requested
⏵ 1	2024-12-02 00:42:24	announced

10.1038/S41467-024-55004-4;

submitter keyword: Fission yeast, Paa1-YFP interactome, PP2A

Sergio Moreno
contact affiliation	IBFG. Instituto de Biologia Funcional y Genomica. CSIC / Salamanca University
contact email	smo@usal.es
lab head
Sergio Moreno
contact affiliation	Instituto de Biología Funcional y Genómica. CSIC / Salamanca University
contact email	smo@usal.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD047703

PRIDE project URI

• PRIDE
  1. PXD047703
     1. Label: PRIDE project
     2. Name: 0518_4 Fission yeast Paa1 interactome