PXD047691-1
PXD047691 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Target identification of dioxygenated amide derivatives interacting proteins |
Description | To identify the direct binding proteins of dioxygenated amide by chemical proteomics, we firstly evaluated the efficiency of optimized probe 6D-PP probe labelling the E. coli bacterial proteomes by in-gel fluorescence. Briefly, the E. coli bacterial lysates were incubated with 100 M probe 6D-PP under 37 °C for 2 h and exposed to 365 nm UV light for 0.5 h to initiate photo-crosslinking. A blank control with only DMSO, a negative control without UV light and a control sample with pretreatment of 500 M D-PP6-C were also included. After conjugation with the TAMRA-N3 by copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry for another 1 h, the probe 6D-PP labelled proteins were separated with 12% SDS-PAGE and imaged by in-gel fluorescence (Fig.3B). The probe 6D-PP probe showed an efficiently UV-dependent and probe 6D-PP specific labelling, supporting it’s readily applicable in ABPP (activity-based protein profiling) experiment. |
HostingRepository | iProX |
AnnounceDate | 2023-12-08 |
AnnouncementXML | Submission_2023-12-10_20:19:35.722.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-10 20:19:16 | ID requested | |
⏵ 1 | 2023-12-10 20:19:36 | announced | |
2 | 2024-02-03 20:56:04 | announced | 2024-02-04: Update information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: antibacterial agents, FabH inhibitory activity |
Contact List
Rui Wang | |
---|---|
contact affiliation | Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
lab head | |
Rui Wang | |
contact affiliation | Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |