PXD047691-1

PXD047691 is an original dataset announced via ProteomeXchange.

Title	Target identification of dioxygenated amide derivatives interacting proteins
Description	To identify the direct binding proteins of dioxygenated amide by chemical proteomics, we firstly evaluated the efficiency of optimized probe 6D-PP probe labelling the E. coli bacterial proteomes by in-gel fluorescence. Briefly, the E. coli bacterial lysates were incubated with 100 M probe 6D-PP under 37 °C for 2 h and exposed to 365 nm UV light for 0.5 h to initiate photo-crosslinking. A blank control with only DMSO, a negative control without UV light and a control sample with pretreatment of 500 M D-PP6-C were also included. After conjugation with the TAMRA-N3 by copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry for another 1 h, the probe 6D-PP labelled proteins were separated with 12% SDS-PAGE and imaged by in-gel fluorescence (Fig.3B). The probe 6D-PP probe showed an efficiently UV-dependent and probe 6D-PP specific labelling, supporting it’s readily applicable in ABPP (activity-based protein profiling) experiment.
HostingRepository	iProX
AnnounceDate	2023-12-08
AnnouncementXML	Submission_2023-12-10_20:19:35.722.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-10 20:19:16	ID requested
⏵ 1	2023-12-10 20:19:36	announced
2	2024-02-03 20:56:04	announced	2024-02-04: Update information.

Dataset with its publication pending

submitter keyword: antibacterial agents, FabH inhibitory activity

Rui Wang
contact affiliation	Shenzhen Bay Laboratory
contact email	wangrui@szbl.ac.cn
lab head
Rui Wang
contact affiliation	Shenzhen Bay Laboratory
contact email	wangrui@szbl.ac.cn
dataset submitter

iProX dataset URI