PXD047669-1

PXD047669 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multi-omics analysis of aggregative multicellularity
Description	The extent to which mRNA and protein levels correlate is still not fully known, especially during development, when cells undergo a major transition in gene expression. One organism with particular development is Dictyostelium discoideum, during which it transitions from free-living unicellular to multicellular. Previously the transcriptome has been thoroughly studied during multicellular development, however the proteome and its correlation to the transcriptome during this transition is not fully understood. In this study, for the first time, paired transcriptomics and proteomics was performed in a time series during aggregative multicellularity. From the analysis, the majority of transcripts were identified as differentially expressed, and we could quantify roughly a third of the proteome during early multicellular development. The proteome and transcriptome correlate relatively highly during steady-state, however this decreases as soon as multicellular aggregation is initiated. Correlation is particularly low at the gene-level during development. We find that dynamically regulated mRNA often leads to linear up- or downregulation of the protein, and that there is a time lag of approximately 2 to 4 hours between mRNA and protein.
HostingRepository	MassIVE
AnnounceDate	2024-03-08
AnnouncementXML	Submission_2024-03-08_05:49:27.818.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bart Edelbroek
SpeciesList	scientific name: Dictyostelium discoideum; NCBI TaxID: 44689;
ModificationList	Oxidation; GG; Carbamidomethyl; Acetyl
Instrument	Q Exactive GC Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-09 12:05:04	ID requested
⏵ 1	2024-03-08 05:49:28	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Multi-omics, Transcriptomics, Proteomics, mRNA-protein correlation, Aggregative multicellularity, Amoebozoa, Dictyostelium discoideum

submitter keyword: Multi-omics, Transcriptomics, Proteomics, mRNA-protein correlation, Aggregative multicellularity, Amoebozoa, Dictyostelium discoideum

Contact List

Fredrik Soderbom
contact affiliation	Uppsala University
contact email	fredrik.soderbom@icm.uu.se
lab head
Bart Edelbroek
contact affiliation	Uppsala University
contact email	bart.edelbroek@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093620/