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PXD047666-1

PXD047666 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of dried blood collected on Mitra devices
DescriptionVenous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. Filtered precipitated were analyzed by direct injection microflow-LC using a Waters ACQUITY, 1 mm x 150 mm ACQUITY Premier column at 100 uL/min with post-column DMSO and solvent divert. MS/MS used an Exploris 480 with staggered/overlapping DIA acquistion. Demultiplexing was performed using HTRMS converter (Biognosys) and data was analyzed with Spectronaut 18 (Biognosys).
HostingRepositoryMassIVE
AnnounceDate2023-12-10
AnnouncementXMLSubmission_2023-12-10_07:50:32.973.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatt Foster
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-08 20:36:02ID requested
12023-12-10 07:50:33announced
Publication List
no publication
Keyword List
submitter keyword: whole blood, microsampling, microflow, data-indepndent acquistion, plasma
Contact List
Matthew W. Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
lab head
Matt Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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