PXD047664-1

PXD047664 is an original dataset announced via ProteomeXchange.

Title	Differential complexome profiling of CRISTA WT and KO HEK293T mitochondria
Description	CRISTA (SMIM26) is a mitochondrial microprotein important for assembly of complex I. Here we used differential complexome profiling to study the composition of ETC complexes in SMIM26 WT and KO HEK293T mitochondria. CRISTA potentiates mitochondrial serine import via direct interactions with the serine transporter SFXN1 in the inner mitochondrial membrane. Its C-terminus furthermore interacts with the mitochondrial ribosome to form a SFXN1-CRISTA-mitoribosome triad that is enriched for mt-ND5 mRNA encoding a key enzymatic subunit of Complex I. Deletion of CRISTA reduces mitochondrial serine uptake, leading to reduced levels of folate intermediates. This in turn impairs 5-taurinomethyl(thio)uridinylation of mitochondrial tRNAs (tm5U and tm5S2U), leading to a specific loss of ND5 translation and a stall in Complex I biogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-06-18
AnnouncementXML	Submission_2025-06-18_00:24:11.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-08 14:47:25	ID requested
⏵ 1	2025-06-18 00:24:12	announced

10.1016/J.MOLCEL.2025.05.033;

submitter keyword: microproteins,Complexome profiling, Complex I, mitochondria, supercomplexes

Lena Ho
contact affiliation	Cardiovascular and Metabolic Diseases, Duke-NUS Medical School, Singapore
contact email	lena@ho-lab.org
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047664
     1. Label: PRIDE project
     2. Name: Differential complexome profiling of CRISTA WT and KO HEK293T mitochondria