PXD047659-1

PXD047659 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Decrypting lysine deacetylase inhibitor action and protein modifications by dose-resolved proteomics
Description	Lysine deacetylase inhibitors (KDACis) are approved for cutaneous T-cell lymphoma (CTCL) and multiple myeloma. Nevertheless, their mechanisms of action (MoA) remain elusive. To characterize the MoA of these drugs in more detail, we systematically measured dose-dependent changes in protein expression, acetylation, and phosphorylation in response to 21 clinical and pre-clinical KDACis. MV4-11 cells were treated for 6 h with vehicle control and 10 increasing doses of the respective drug (from 100 pM to 30 mM). PTM-carrying and unmodified peptides were analyzed separately by liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide and protein identification and quantification. Furthermore, time-dependent experiments were carried out for Vorinostat and Panobinostat at their respective pEC50 concentrations from the cell viability data. In an independent experiment, the subcellular proteomes of Panobinostat-treated cells were measured.
HostingRepository	MassIVE
AnnounceDate	2024-04-21
AnnouncementXML	Submission_2024-04-21_08:46:00.652.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yun-Chien Chang
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; Phospho
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-08 10:19:32	ID requested
⏵ 1	2024-04-21 08:46:01	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: HDACs, Lysine deacetylase inhibitors, Acetylome, Phosphoproteome, Proteomic pharmacology, Chemical proteomics, Dose-dependent response

submitter keyword: HDACs, Lysine deacetylase inhibitors, Acetylome, Phosphoproteome, Proteomic pharmacology, Chemical proteomics, Dose-dependent response

Contact List

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	kuster@tum.de
lab head
Yun-Chien Chang
contact affiliation	Chair of proteomics and bioanalytics, TUM
contact email	yunchien.chang@tum.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093608/