PXD047650-2

PXD047650 is an original dataset announced via ProteomeXchange.

Title	Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: Not as effective as cells
Description	Although the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative effects on tissue repair, the potential of MSC secretomes as effective substitutes for cellular therapies remains underexplored. In this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efficacy for skin wound healing using a translationally-relevant murine model. Proteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specific proteins associated with wound healing and angiogenesis were differentially expressed. Despite a similar angiogenic potential in vivo, DSC- and ASC-secretomes were found to be less effective than cells in accelerating wound closure and promoting tissue remodeling. Overall, secretome-treated groups showed intermediary results between cells and control (empty scaffold) treated groups. These findings highlight that although secretomes possess therapeutic potential, their efficacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and offers insights into their potential applications in regenerative medicine.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:27.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Augusto Stimamiglio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-08 07:33:33	ID requested
1	2023-12-22 08:18:56	announced
⏵ 2	2024-10-22 06:18:28	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: exosomes, Integra Matrix, proteomics, dermis,MSC, conditioned medium

Helena Zomer
contact affiliation	Department of Physiological Sciences, College of Veterinary Medicine, University of Florida
contact email	helenazomer@ufl.edu
lab head
Marco Augusto Stimamiglio
contact affiliation	Fiocruz
contact email	marco.stimamiglio@fiocruz.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047650
     1. Label: PRIDE project
     2. Name: Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: Not as effective as cells