PXD047608-1

PXD047608 is an original dataset announced via ProteomeXchange.

Title	FERONIA-mediated CC1 phosphorylation dynamically regulates cortical microtubule behavior and salt stress responses in Arabidopsis
Description	Cell wall remodeling is important for plants to adapt to environmental stress, and therefore needs to be efficiently modulated during stress conditions. Under salt stress, cortical microtubules undergo a depolymerization-reassembly process to promote the biosynthesis of stress-adaptive cellulose, but the regulatory mechanisms underlying this process are still largely unknown. In this study, we reveal that FERONIA, a potential cell wall sensor, interacts with CC1 and its closest homolog, CC2; two proteins that are required for cortical microtubule reassembly under salt stress. Biochemical data indicate that FER phosphorylates CC1 on multiple residues in the second and third hydrophobic microtubule-binding regions in vitro and in vivo, and that these phosphorylations impact the ability of CC1 to engage with microtubules. Furthermore, FER kinase activity and the CC1 phosphorylation level were temporally coordinated upon exposure to salt stress, which coincided with dynamic microtubule reorganization. Both fer-4 and cc1 cc2 mutants displayed similar salt-sensitive phenotypes, which were related to compromised microtubule reassembly, corroborating that FER and CC1/CC2 function in the same pathway to regulate microtubule organization. Taken together, our study outlines an important intracellular mechanism that maintains microtubule dynamics during salt exposure in plant cells.
HostingRepository	PRIDE
AnnounceDate	2024-08-30
AnnouncementXML	Submission_2024-08-30_01:35:15.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunzhao Zhao
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-07 03:44:34	ID requested
⏵ 1	2024-08-30 01:35:15	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, LC-MS, FERONIA, CC1

Chunzhao Zhao
contact affiliation	Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
contact email	czzhao@cemps.ac.cn
lab head
Chunzhao Zhao
contact affiliation	CAS Center for Excellence in Molecular Plant Sciences
contact email	czzhao@psc.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD047608

PRIDE project URI

• PRIDE
  1. PXD047608
     1. Label: PRIDE project
     2. Name: FERONIA-mediated CC1 phosphorylation dynamically regulates cortical microtubule behavior and salt stress responses in Arabidopsis