PXD047581-1

PXD047581 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics analysis of nuclear and mitochondrial from human coronary artery endothelial cells fraction treated with calcioprotein particles
Description	Calciprotein particles (CPPs) are indispensable scavengers of excessive Ca2+ and PO43- ions in the blood, being internalized and recycled by liver and spleen macrophages, monocytes, and endothelial cells (ECs). Despite the fact, that CPPs are associated with many cardiovascular diseases and are known to trigger endothelial dysfunction, the exact mechanisms of its recycling by ECs are still poorly studied. Particularly, because of the low resolution of the standard proteomics approach. We assume that CPPs internalization would cause oxidative stress and strong rearrangements in the transcription. Therefore, we performed a shotgun proteomics analysis of nuclear and mitochondrial fraction of human coronary artery endothelial cells (HCAEC) treated with primary (amorphous) or secondary (crystalline) CPPs (CPP-P and CPPs, respectively). Contact: Dr Anton G. Kutikhin, Laboratory of Molecular, Translational and Digital Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russia (lab head).
HostingRepository	PRIDE
AnnounceDate	2023-12-31
AnnouncementXML	Submission_2023-12-30_22:11:17.811.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Repkin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-06 11:50:20	ID requested
⏵ 1	2023-12-30 22:11:18	announced

10.3390/JCDD11010005;

submitter keyword: Calciprotein particles, TimsToF Pro, DDA-PASEF, mitochondria, endothelium, nucleus, subcellular fractionation

Dr. Anton G. Kutikhin
contact affiliation	Laboratory of Molecular, Translational and Digital Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russia
contact email	antonkutikhin@gmail.com
lab head
Egor Repkin
contact affiliation	Saint-Petersburg State University
contact email	erepkin53@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047581
     1. Label: PRIDE project
     2. Name: Shotgun proteomics analysis of nuclear and mitochondrial from human coronary artery endothelial cells fraction treated with calcioprotein particles