PXD047444-1

PXD047444 is an original dataset announced via ProteomeXchange.

Title	Function and relevance of sushi domain-containing protein 2 (SUSD2) in circulating tumor cells of metastatic estrogen receptor positive breast cancer
Description	Albeit responding well to therapy, estrogen receptor positive breast cancers frequently become subsequently resistant. This leads to distant metastasis and release of circulating tumor cells (CTCs) into the blood stream and ends with the death of the patient. Here we analyzed the proteome of CTC-ITB-01 by mass spectrometry and quantified 7954 different proteins using MCF-7 as a reference proteome. The data were deposited in a database for public access. CTC-ITB-01 is a CTC cell line derived from a patient with metastatic and estrogen receptor alpha (ER-alpha) positive breast cancer. We found remarkable high levels of the protein sushi domain-containing protein 2 (SUSD2) in CTC-ITB-01. Presence of SUSD2 in CTC of patients with breast cancer was confirmed by CellSearch (9/23 CTC positive cases). Ectonucleotide pyrophosphatase /phosphodiesterase family member 1 (ENPP1) was detected in CTC-ITB-01 and confirmed in CTC of breast cancer patients (n=3). ENPP1 can mediate immune evasion of tumor cells. Overexpression of SUSD2 in MCF-7 revealed upregulation of ER-alpha, 78 kDa glucose-regulated protein (GRP78) downregulation of programmed cell death protein 4 (PDCD4). GRP78 mediates resistance to chemotherapy, whereas loss of PDCD4 is involved in drug resistance and malignant transformation. Database search revealed significantly reduced overall survival in luminal breast cancer for low PDCD4 levels (n=348; p=0.0323). In conclusion, high levels of SUSD2 may confer improved cytoprotection of metastatic breast cancer cells and their CTC travelers to distant organs in the blood.
HostingRepository	PRIDE
AnnounceDate	2025-02-04
AnnouncementXML	Submission_2025-02-04_06:38:59.753.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Bartkowiak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-01 06:41:33	ID requested
⏵ 1	2025-02-04 06:39:00	announced

10.1038/S41598-025-87122-4;

submitter keyword: breast cancer,SUSD2, estrogen receptor positive, circulating tumor cells, metastasis

Kai Bartkowiak
contact affiliation	University Medical Center Hamburg-Eppendorf, Department of Tumor Biology, Germany
contact email	kbartkowiak@uke.de
lab head
Kai Bartkowiak
contact affiliation	Department of Tumor Biology University Medical Center Hamburg-Eppendorf
contact email	kbartkowiak@uke.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047444
     1. Label: PRIDE project
     2. Name: Function and relevance of sushi domain-containing protein 2 (SUSD2) in circulating tumor cells of metastatic estrogen receptor positive breast cancer