PXD047419-1

PXD047419 is an original dataset announced via ProteomeXchange.

Title	Decrypting lysine deacetylase inhibitor action and protein modifications by dose-resolved proteomics
Description	Lysine deacetylase inhibitors (KDACis) are approved drugs for cutaneous T-cell lymphoma (CTCL) and multiple myeloma. The drugs lead to increasing acetylation levels of histones and other proteins, altered gene expression, and cell death. To characterize the mechanisms of action of these drugs in more detail, we systematically measured dose-dependent drug-induced changes in protein expression, acetylation, and phosphorylation in response to 21 clinical and pre-clinical KDACis. The resulting 872,000 dose-response curves revealed, for instance, poor selectivity of HDAC1, 2, 3, and 6 inhibitors in cells, strong cross-talk between acetylation and phosphorylation pathways, localization of most drug-responsive acetylation sites to intrinsically disordered regions (IDRs), an underappreciated role of acetylation in protein structure and a shift in EP300 protein abundance between the cytoplasm and the nucleus. This comprehensive dataset serves as a resource for the investigation of the molecular mechanisms underlying KDACi action in cells and can be explored online in ProteomicsDB.
HostingRepository	PanoramaPublic
AnnounceDate	2024-04-21
AnnouncementXML	Submission_2024-04-21_22:42:29.356.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Miriam Abele
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Acetyl; Amidated; Oxidation
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-30 14:28:38	ID requested
⏵ 1	2024-04-21 22:42:29	announced

Dataset with its publication pending

submitter keyword: Chemical proteomics, acetylation, phosphorylation, lysine deacetylase inhibitors, HDACs, proteomic pharmacology

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany
contact email	kuster@tum.de
lab head
Miriam Abele
contact affiliation	Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany
contact email	m.abele@tum.de
dataset submitter

Panorama Public dataset URI